A gene encoding a 69-kilodalton major surface protein of Giardia intestinalis trophozoites
| Autor: | Peter L. Ey, Paul A. Manning, Kumkum Khanna, Graham Mayrhofer |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Untranslated region
Sequence analysis Genes Protozoan Molecular Sequence Data Protozoan Proteins Biology medicine.disease_cause Homology (biology) Conserved sequence medicine Animals Giardia lamblia Amino Acid Sequence RNA Messenger Molecular Biology Gene Genetics Base Sequence Sequence Homology Amino Acid Intron Nucleic acid sequence Chromosome Mapping DNA Protozoan Molecular biology Molecular Weight Antigens Surface Parasitology RNA Protozoan |
| Zdroj: | Molecular and Biochemical Parasitology. 58:247-257 |
| ISSN: | 0166-6851 |
| Popis: | A gene encoding a 68.5-kDa trophozoite surface protein (TSP11) of the Australian Giardia intestinalis (syn. G. lamblia) isolate, Ad-1, has been cloned from a genomic expression library screened with an antiserum specific for 3 major surface antigens. Sequence analysis of two overlapping genomic fragments identified a single open reading frame that contained no introns and predicted a cysteine-rich, 667-residue polypeptide with features common to other trophozoite surface proteins. These include the presence of 27 copies of the 4-amino acid Cys-X-X-Cys motif, an N-terminal signal sequence and a highly conserved, hydrophobic C-terminal segment. Transcripts from the tsp11 gene were detected as a single band on Northern blots using total RNA extracted from Ad-1 trophozoites. Primer extension analysis indicated that the mRNA has a 5‘ untranslated region of only 5 nt, similar to the very short (1–6 nt) leader sequences reported for other Giardia mRNAs. A large portion of the promoter distal segment of tsp1 has homology with tsa417, a gene encoding a 72.5-kDa trophozoite surface antigen of the Afghanistan-derived G. intestinalis isolate, WB [13]. |
| Databáze: | OpenAIRE |
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