Excito-repellency and biological safety of β-caryophyllene oxide against Aedes albopictus and Anopheles dirus (Diptera: Culicidae)

Autor: Chutipong Sukkanon, Evelyne Ollivier, Sylvie Manguin, Jirod Nararak, Valérie Mahiou-Leddet, Carole Di Giorgio, Theeraphap Chareonviriyaphap
Přispěvatelé: Kasetsart University - KU (THAILAND), Kasetsart University (KU), Hydrosciences Montpellier (HSM), Institut national des sciences de l'Univers (INSU - CNRS)-Institut de Recherche pour le Développement (IRD)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UMR237-Aix Marseille Université (AMU)-Avignon Université (AU), Thailand Research Fund through the International Research Network (Grant No. IRN58W0003-IRN5803PHDW04). French Ministry of Foreign Affairs (MEAE) through the Bio-Asie programme (BioVectrol project) and the PHC Siam (BioVecThai project, Campus France No 33765NG), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Acta Tropica
Acta Tropica, Elsevier, 2020, 210, pp.105556. ⟨10.1016/j.actatropica.2020.105556⟩
Acta Tropica, 2020, 210, pp.105556. ⟨10.1016/j.actatropica.2020.105556⟩
ISSN: 0001-706X
DOI: 10.1016/j.actatropica.2020.105556⟩
Popis: The activity of β-caryophyllene oxide as either a contact or noncontact repellent was evaluated against two laboratory strains (Aedes albopictus and Anopheles dirus) using an excito-repellency test system. N, N-Diethyl-3-methylbenzamide (DEET) was used as a standard reference baseline for comparative purposes. β-Caryophyllene oxide and DEET were tested at concentrations of 0.1, 0.25, 0.5 and 1.0% (v/v). In addition, the phototoxic and genotoxic effects of β-caryophyllene oxide were investigated on Balb/c 3T3 mouse fibroblasts (3T3-L1) and Chinese hamster ovary cell line (CHO-K1). The results demonstrated that the higher concentrations of test compounds (0.5 and 1.0%) produced greater behavioral responses. Aedes albopictus was more sensitive to β-caryophyllene oxide than An. dirus. Moderate avoidance response rates (25-56% escape) of Ae. albopictus at 0.5% and 1.0% β-caryophyllene oxide were observed in contact and noncontact trials compared with low response rates from An. dirus (26-31% escape). DEET at ≤1% displayed lower irritancy and repellency (1-38%) than β-caryophyllene oxide when tested against the two mosquito species. Knockdown responses (37%) were only observed in An. dirus exposed to 1% β-caryophyllene oxide in the contact trial. β-Caryophyllene oxide did not show any phototoxic potential (PIF= 0.38) nor was there any significant genotoxic response as indicated by no increase in micro-nucleated cells with or without metabolic activation. β-Caryophyllene oxide could be considered as a safe repellent, effective against mosquitoes.
Databáze: OpenAIRE
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