Amperometric cholesterol biosensor based on reconstituted cholesterol oxidase on boronic acid functional conducting polymers
| Autor: | Mehmet Şenel, Muamer Dervisevic, Cevdet Nergiz, Emre Cevik, M. Fatih Abasıyanık |
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| Rok vydání: | 2016 |
| Předmět: |
inorganic chemicals
Flavin adenine dinucleotide Conductive polymer Chromatography Cholesterol oxidase General Chemical Engineering 010401 analytical chemistry technology industry and agriculture 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences Combinatorial chemistry Amperometry 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound chemistry Electrode Monolayer Electrochemistry lipids (amino acids peptides and proteins) 0210 nano-technology Biosensor Boronic acid |
| Zdroj: | Journal of Electroanalytical Chemistry. 776:18-24 |
| ISSN: | 1572-6657 |
| Popis: | An amperometric cholesterol biosensor was fabricated by electropolymerization method of conducting polymers on pencil graphite electrode (PGE) after which apo-cholesterol oxidase (apo-ChOx) was immobilized onto a flavin adenine dinudeotide (FAD) monolayer by reconstitution method. In this study two well-known and widely used materials in the field of biosensors thiophene-3-boronic acid and 3-aminophenyl boronic acid were electropolymerized onto PGE and modified with cholesterol oxidase. Electrochemical performance of modified electrodes and their properties were compared. Based on the results, thiephene-3-boronic acid based electrode shows sensitivity 10 times higher response than that of one based on 3-aminophenyl boronic add. The effect of pH and temperature were assessed as well the reusability and stability of the fabricated biosensor were discussed. (C) 2016 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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