Spatial control and cell adhesion selectivity on model gold surfaces grafted with elastin-like recombinamers
| Autor: | J. Carlos Rodríguez-Cabello, I. González de Torre, Luis G. Quintanilla, María J. Alonso, Tatjana Flora |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Polymers and Plastics
General Physics and Astronomy Biofuncionalización superficial 02 engineering and technology 010402 general chemistry 01 natural sciences Contact angle Ablación láser Materials Chemistry Surface bio-functionalization Cell adhesion chemistry.chemical_classification Spatial control Adhesión celular Organic Chemistry Adhesion Quartz crystal microbalance Recombinantes tipo elastina 021001 nanoscience & nanotechnology Grafting Laser ablation 0104 chemical sciences 3. Good health Elastin-like recombinamers chemistry Control espacial Thiol Biophysics 0210 nano-technology Selectivity Cysteine |
| Zdroj: | UVaDOC: Repositorio Documental de la Universidad de Valladolid Universidad de Valladolid European Polymer Journal UVaDOC. Repositorio Documental de la Universidad de Valladolid instname |
| ISSN: | 0014-3057 |
| DOI: | 10.1016/j.eurpolymj.2018.06.026 |
| Popis: | Producción Científica A simple system for cell selectivity and spatially controlled cell adhesion has been developed using model gold surfaces grafted with a combination of two ELRs containing into their backbone cell-adhesion domains such as RGD and REDV. Grafting onto gold was achieved via redox reaction through thiol groups present in amino terminal cysteine tails of the ELRs. The correlation among contact angle, SEM micrographs, AFM, XPS and QCM-D have been carried out. After in-depth adhesion studies, a mixture of 75% ELR-REDV and 25% ELR-RGD was found to exhibit high selectivity for endothelial cells, promoting strong adhesion thereof. Consequently, certain areas of gold surfaces (strips) were cleaned by laser ablation and functionalized with the mixture 75% ELR-REDV - 25% ELR-RGD leading to a spatial segregation of the co-culture made of HUVEC and HFF1 cells. This platform therefore exhibits selective spatial control over cell adhesion associated with the bioactive epitopes (RGD and REDV) contained in the ELR sequence, since each functionalized surface (including strips) have similar topographic, hydrophobic and mechanical properties. 2020-09 Comisión Europea (NMP-2014-646075, MSCA-ITN-2014-642687) Ministerio de Economía, Industria y Competitividad (PCIN-2015-010, MAT2015-68901-R, MAT2016-78903-R and MAT2016-79435-R) Junta de Castilla y León (programa de apoyo a proyectos de investigación - Ref. Project VA015U16) Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y León |
| Databáze: | OpenAIRE |
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