Pathogen displacement during intermittent catheter insertion: a novel in vitro urethra model
| Autor: | Morgan Tierney, Declan M. Devine, Andrew M. Fogarty, Victoria E. Wagner, David Scully, Yvonne J. Cortese |
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| Rok vydání: | 2019 |
| Předmět: |
Male
medicine.medical_specialty Intermittent catheter Urinary system Urology Urinary Catheters Models Biological Applied Microbiology and Biotechnology 03 medical and health sciences Urethra medicine Humans Displacement (orthopedic surgery) Intermittent Urethral Catheterization Pathogen 030304 developmental biology 0303 health sciences Catheter insertion Bacteria 030306 microbiology business.industry General Medicine In vitro Catheter medicine.anatomical_structure Catheter-Related Infections Urinary Tract Infections Equipment Contamination business Biotechnology |
| Zdroj: | Journal of Applied Microbiology. 128:1191-1200 |
| ISSN: | 1365-2672 1364-5072 |
| DOI: | 10.1111/jam.14533 |
| Popis: | Aim To develop a novel in vitro urethra model and use it to determine if insertion of an intermittent urinary catheter (IC) displaces pathogenic bacteria from the urethral meatus along the urethra. Methods Displacement of microbial growth after catheter insertion was assessed using a novel in vitro urethra model. The in vitro urethra model utilized chromogenic agar and was inoculated with bacteria at one side of the artificial urethra channel, to act as a contaminated urethral meatus, before an IC was inserted into the channel. Three ICs types were used to validate the in vitro urethra model and methodology. Results When compared to the bacterial growth control, a significant difference in bacterial growth was found after insertion of the uncoated (P ≤ 0·001) and hydrophilic coated (P ≤ 0·009) catheters; no significant difference when a prototype catheter was inserted into the in vitro urethra model with either bacterial species tested (P ≥ 0·423). Conclusion The results presented support the hypothesis that a single catheter insertion can initiate a catheter-associated urinary tract infection. Significance and impact of the study The in vitro urethra model and associated methodology were found to be reliable and reproducible (P ≥ 0·265) providing new research tool for the development and validation of emerging technologies in urological healthcare. |
| Databáze: | OpenAIRE |
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