Summary of reference chemicals evaluated by the fish short‐term reproduction assay, OECD TG229, using Japanese Medaka, Oryzias latipes
| Autor: | Yuta Onishi, Atsushi Sawai, Haruna Watanabe, Tomomi Sato, Hidenori Ishikawa, Jun Yamamoto, Masaaki Koshio, Kunihiko Yamazaki, Yukio Kawashima, Taisen Iguchi, Tetsuro Okamura, Norihisa Tatarazako |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Oryzias Estrogen receptor 010501 environmental sciences Endocrine Disruptors Toxicology 01 natural sciences OECD TG229 Andrology Japanese medaka 03 medical and health sciences Vitellogenin In vivo Toxicity Tests Androgen Receptor Antagonists Animals Research Articles 030304 developmental biology 0105 earth and related environmental sciences 0303 health sciences Oryzias latipes biology Reproduction Estrogens Japanese Medaka biology.organism_classification Androgen receptor endocrine disrupting effect Toxicity biology.protein Androgens Biological Assay Female Estrogen Receptor Antagonists Receptors Progesterone Estrogen receptor alpha Research Article |
| Zdroj: | Journal of Applied Toxicology |
| ISSN: | 1099-1263 0260-437X |
| Popis: | Under the Organisation for Economic Co‐operation and Development (OECD), the Ministry of the Environment of Japan (MOE) added Japanese medaka (Oryzias latipes) to the test guideline fish short‐term reproduction assay (FSTRA) developed by the United States Environmental Protection Agency (US EPA) using fathead minnow (Pimephales promelas). The FSTRA was designed to detect endocrine disrupting effects of chemicals interacting with the hypothalamic–pituitary–gonadal axis (HPG axis) such as agonists or antagonists on the estrogen receptor (Esr) and/or the androgen receptor (AR) and steroidogenesis inhibitors. We conducted the FSTRA with Japanese medaka, in accordance with OECD test guideline number 229 (TG229), for 16 chemicals including four Esr agonists, two Esr antagonists, three AR agonists, two AR antagonists, two steroidogenesis inhibitors, two progesterone receptor agonists, and a negative substance, and evaluated the usability and the validity of the FSTRA (TG229) protocol. In addition, in vitro reporter gene assays (RGAs) using Esr1 and ARβ of Japanese medaka were performed for the 16 chemicals, to support the interpretation of the in vivo effects observed in the FSTRA. In the present study, all the test chemicals, except an antiandrogenic chemical and a weak Esr agonist, significantly reduced the reproductive status of the test fish, that is, fecundity or fertility, at concentrations where no overt toxicity was observed. Moreover, vitellogenin (VTG) induction in males and formation of secondary sex characteristics (SSC), papillary processes on the anal fin, in females was sensitive endpoints to Esr and AR agonistic effects, respectively, and might be indicators of the effect concentrations in long‐term exposure. Overall, it is suggested that the in vivo FSTRA supported by in vitro RGA data can adequately detect effects on the test fish, O. latipes, and probably identify the mode of action (MOA) of the chemicals tested. Fish short‐term reproduction assay (OECD, TG229) with Japanese medaka (Oryzias latipes) was conducted for 16 reference chemicals including 4 estrogen receptor (Esr) agonists, 2 Esr antagonists, 3 androgen receptor (AR) agonists, 2 AR antagonists, 2 steroidogenesis inhibitors, 2 progesterone receptor agonists, and a negative substance, and confirmed the usability and the validity of the TG229 protocol. |
| Databáze: | OpenAIRE |
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