Assessment of cell-substrate adhesion by a centrifugal method

Autor:	William S. Ramsey, E. D. Nowlan, W. Hertl
Rok vydání:	1984
Předmět:	Centrifugal force Time Factors Centrifugation Plant Science Anatomy Adhesion Biology Kidney Cell Line law.invention Cell culture law Chlorocebus aethiops Cell Adhesion Microscopy Electron Scanning Biophysics Animals Electron microscope Cell adhesion Cell-substrate adhesion Filopodia Biotechnology
Zdroj:	In Vitro. 20:796-801
ISSN:	1475-2689 0073-5655
DOI:	10.1007/bf02618295
Popis:	A centrifugal method has been evaluated for measuring the strength of Vero Green Monkey kidney cell adhesion to growth surfaces. The centrifugal force necessary to remove cells gave a quantitative measure of cell adhesion and hence the quality of the growth surface. After being subjected to high gravity forces, both the remaining attached cells and the detached cells were viable, indicating the detachment process did not simply rupture the cell. Electron microscope examination of growth surfaces after cell detachment suggested that remnants related to filopodia remained.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::224ff0be113c492bd123bacd686c0449 https://doi.org/10.1007/bf02618295 Zobrazit plný text záznamu Full text from SpringerLink