| Popis: |
The current study aimed to detect the presence of Entomopathogenic nematodes (EPNs) and isolate them from soils of some areas of Diyala Governorate, Iraq. The isolates were molecularly identified, and the results showed, depending on the symptoms of infection, the color of the infected large wax larvae, and some taxonomic morphological characteristics, that all isolates were of the genus Steinernema, and the molecular identification, compared to the isolates recorded in the global gene bank, showed that the isolates belong to the S. carpocapsea type. Nematodes were used experimentally to study their efficiency in controlling the cucurbit fruit fly insect, and the results of the pathogenicity study of the local and commercial S. carpocapsea species on cucurbit fruit fly larvae confirmed that the local isolation is very effective in causing death on the last stage larvae of cucurbit fruit fly under laboratory conditions. Three concentrations of 25, 50, and 100 larvae/IJS were used for each of the local and commercial isolates. The death rate after three days of experimental treatment with the local isolate for the last stage larvae of the cucurbit fruit fly reached 50, 66, 56, 33, and 83%, respectively. In commercial isolates, the death rates reached to 66.66, 86.66, and 96.66%, respectively, these were compared with the control treatment, and there were significant differences among the concentrations of each type. the LSD values for the local isolate was 8.74 and for the commercial 8.05, and significant differences appeared between the local and commercial types during each concentration, and the value of LSD for each of the concentrations 25, 50, 100 / 5.94, 7.06, 6.88 larvae /IJS, respectively, and the value of LC50 reached 31,454 larvae / IJS. |
