Microbial fed-batch production of 1,3-propanediol by Klebsiella pneumoniae under micro-aerobic conditions
| Autor: | Xi Chen, W.-T. Qi, Ping Xu, S.-J. Gao, Daijia Zhang, Zhilong Xiu |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Glycerol
General Medicine Bacterial growth Biology Applied Microbiology and Biotechnology Aerobiosis Culture Media Propanediol Klebsiella pneumoniae chemistry.chemical_compound Bioreactors chemistry Biochemistry Propylene Glycols Fermentation Aerobie Anaerobiosis 1 3-Propanediol Food science Anaerobic exercise Mixed acid fermentation Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 63:143-146 |
| ISSN: | 1432-0614 0175-7598 |
| Popis: | The microbial production of 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae under micro-aerobic conditions was investigated in this study. The experimental results of batch fermentation showed that the final concentration and yield of 1,3-PD on glycerol under micro-aerobic conditions approached values achieved under anaerobic conditions. However, less ethanol was produced under microaerobic than anaerobic conditions at the end of fermentation. The batch micro-aerobic fermentation time was markedly shorter than that of anaerobic fermentation. This led to an increment of productivity of 1,3-PD. For instance, the concentration, molar yield, and productivity of 1,3-PD of batch micro-aerobic fermentation by K. pneumoniae DSM 2026 were 17.65 g/l, 56.13%, and 2.94 g l(-1) h(-1), respectively, with a fermentation time of 6 h and an initial glycerol concentration of 40 g/l. Compared with DSM 2026, the microbial growth of K. pneumoniae AS 1.1736 was slow and the concentration of 1,3-PD was low under the same conditions. Furthermore, the microbial growth in fed-batch fermentation by K. pneumoniae DSM 2026 was faster under micro-aerobic than anaerobic conditions. The concentration, molar yield, and productivity of 1,3-PD in fed-batch fermentation under micro-aerobic conditions were 59.50 g/l, 51.75%, and 1.57 g l(-1) h(-1), respectively. The volumetric productivity of 1,3-PD under microaerobic conditions was almost twice that of anaerobic fed-batch fermentation, at 1.57 and 0.80 g l(-1) h(-1), respectively. |
| Databáze: | OpenAIRE |
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