Clusterin transcript variants expression in thyroid tumor: A potential marker of malignancy?
Autor: | Elda Perlino, Anna Ciampolillo, Nicoletta Nuzziello, Angela Pezzolla, Eugenio Maiorano, Serafina Lattarulo, Paolo Fuzio, Anna Napoli, Francesco Giorgino, Sabino Liuni |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2015 |
Předmět: |
Thyroid nodules
Adult Male Pathology medicine.medical_specialty Cancer Research Thyroid Gland CLU Thyroid tumour Gene expression Biopsy medicine Biomarkers Tumor Genetics Humans Protein Isoforms Neoplastic transformation RNA Messenger Thyroid Neoplasms Thyroid cancer Aged medicine.diagnostic_test Clusterin biology business.industry Thyroid Middle Aged medicine.disease Carcinoma Papillary 3. Good health medicine.anatomical_structure Oncology biology.protein Immunohistochemistry Female business Research Article |
Zdroj: | BMC cancer (Online) 15 (2015). doi:10.1186/s12885-015-1348-0 info:cnr-pdr/source/autori:Fuzio, Paolo; Napoli, Anna; Ciampolillo, Anna; Lattarulo, Serafina; Pezzolla, Angela; Nuzziello, Nicoletta; Liuni, Sabino; Giorgino, Francesco; Maiorano, Eugenio; Perlino, Elda/titolo:Clusterin transcript variants expression in thyroid tumor: A potential marker of malignancy?/doi:10.1186%2Fs12885-015-1348-0/rivista:BMC cancer (Online)/anno:2015/pagina_da:/pagina_a:/intervallo_pagine:/volume:15 BMC Cancer |
Popis: | Background: Clusterin (CLU) is a ubiquitous multifunctional factor involved in neoplastic transformation. The CLU transcript variants and protein forms play a crucial role in balancing cells proliferation and death. Methods: We investigated the regulation of CLU transcript variants expression in an in vivo model system consisting of both neoplastic tissues and fine needle aspiration biopsy (FNAB) samples isolated from patients undergoing thyroidectomy. Results: The immunohistochemical analyses showed an overall CLU up-regulation in papillary carcinoma. A specific CLU2 transcript variant increase was registered using qPCR in papillary carcinomas while CLU1 decreased. In addition, the analysis of CLU transcripts expression level showed an increase of the CLU2 transcript in the TIR 3 patients with histologically confirmed thyroid cancer. Conclusions: Our results suggest the existence of a specific alteration of CLU2:CLU1 ratio towards CLU2, thus providing the first circumstantial evidence for the potential use of CLU transcript variants as effective biomarkers for a more accurate assessment of the so called "indeterminate" thyroid nodules. |
Databáze: | OpenAIRE |
Externí odkaz: |