Biphasic effect of oleic acid on hepatic cholesterogenesis
| Autor: | Henry G. Wilcox, Murray Heimberg, Lauren M. Cagen, Thomas V. Fungwe |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Male
medicine.medical_specialty Biophysics Oleic Acids In Vitro Techniques Biology Reductase Biochemistry Gene Expression Regulation Enzymologic chemistry.chemical_compound Internal medicine medicine Animals Bovine serum albumin Molecular Biology Triglycerides Triglyceride Cholesterol Fatty Acids Albumin Cell Biology Rats Perfusion Oleic acid Endocrinology Liver chemistry HMG-CoA reductase biology.protein Hydroxymethylglutaryl CoA Reductases lipids (amino acids peptides and proteins) Oleic Acid |
| Zdroj: | Biochemical and Biophysical Research Communications. 183:21-26 |
| ISSN: | 0006-291X |
| Popis: | Livers isolated from adult male rats were perfused in vitro with oleic acid (0.6 mM) as a complex with bovine serum albumin. Albumin alone was infused in control experiments. Oleic acid exerted a biphasic effect on incorporation of 3H2O into cholesterol, which was inhibitory during the first hour of perfusion, but exhibited a net stimulatory effect over a four hour period. No differences were observed in total activity or apparent phosphorylation state of HMG-CoA reductase after one hour of perfusion, with or without addition of oleic acid, implying that some other step limits the rate of cholesterol synthesis during this interval. After four hours of perfusion, HMG-CoA reductase activity was higher in livers perfused with oleic acid than in those perfused in its absence, in agreement with the observed differences in rates of cholesterol synthesis. |
| Databáze: | OpenAIRE |
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