Fluorescence Quenching of the Europium Tetracycline Hydrogen Peroxide Complex by Copper (II) and other Metal Ions
| Autor: | Luis Fermín Capitán Vallvey, Michael Schäferling, Otto S. Wolfbeis, Clara Cano-Raya, Maria Dolores Fernandez Ramos |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Aqueous solution
Quenching (fluorescence) Metal ions in aqueous solution 010401 analytical chemistry Analytical chemistry chemistry.chemical_element 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences Copper Fluorescence 0104 chemical sciences chemistry.chemical_compound chemistry 0210 nano-technology Hydrogen peroxide Europium Luminescence Instrumentation Spectroscopy |
| Zdroj: | Applied Spectroscopy. 59:1209-1216 |
| ISSN: | 1943-3530 0003-7028 |
| DOI: | 10.1366/000370205774430945 |
| Popis: | The europium–tetracycline complex [Eu(Tc)] is known to show only weak fluorescence with an emission maximum at 615 nm. On addition of hydrogen peroxide (HP), the strongly fluorescent [Eu(Tc)(HP)] complex is formed, which displays a 15-fold stronger luminescence intensity. This study describes the decrease in luminescence intensity of the [Eu(Tc)(HP)] complex in aqueous solution in the presence of Cu2+, Fe3+, Ag+, Al3+, Zn2+, Co2+, Ni2+, Mn2+, Ca2+, and Mg2+. Static and dynamic quenching can be induced by Cu2+, and these processes were quantified by means of their quenching constants. Stern–Volmer plots were also derived from lifetime imaging measurements accomplished by the rapid lifetime determination (RLD) technique based on microwell plate assays, and also by the time-correlated single photon counting (TCSPC) technique. According to those data, a time-resolved fluorescent method for copper determination can be proposed that is based on dynamic quenching of the [Eu(Tc)(HP)] complex by Cu2+ ions. The response to copper concentrations is linear up to 1.6 μmol L−1, providing a detection limit of 0.2 μmol L−1. |
| Databáze: | OpenAIRE |
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