Expression of Inducible Microsomal Prostaglandin E Synthase in Local Lesions of Endometriosis Patients
| Autor: | Norimichi Nemoto, Satoshi Hayakawa, Masahiko Sugitani, Tatsuo Yamamoto, Kenji Sugita, Fumihisa Chishima, Miki Karasaki-Suzuki |
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| Rok vydání: | 2007 |
| Předmět: |
Adult
medicine.medical_specialty Immunology Endometriosis Gene Expression Prostaglandin Prostaglandin E synthase Endometrium chemistry.chemical_compound Microsomes Internal medicine Gene expression medicine Humans Immunology and Allergy RNA Messenger Prostaglandin E2 Menstrual Cycle Prostaglandin-E Synthases Messenger RNA biology Reverse Transcriptase Polymerase Chain Reaction Membrane Proteins Obstetrics and Gynecology Epithelial Cells medicine.disease Immunohistochemistry Molecular biology Intramolecular Oxidoreductases Endocrinology Reproductive Medicine chemistry Cyclooxygenase 2 biology.protein Female lipids (amino acids peptides and proteins) Cyclooxygenase Stromal Cells medicine.drug |
| Zdroj: | American Journal of Reproductive Immunology. 57:218-226 |
| ISSN: | 1600-0897 1046-7408 |
| Popis: | Problem Recently, an inducible microsomal human prostaglandin E synthase (mPGES) was identified. This enzyme converts the cyclooxygenase (COX) product, prostaglandin (PG) H2, to PGE2, an eicosanoid linked to carcinogenesis. Although elevated levels of PGE2 have been observed in many tumor types including colorectal adenomas and cancers, its role in the pathophysiology of endometriosis is unknown. We previously reported increased expression of COX-2 messenger RNA (mRNA) in local lesions of endometriosis. To further elucidate the mechanism responsible for the elevated levels of PGE2 in endometriosis, we examined the expression levels of mPGES. Method of study Samples were obtained from 28 patients, fixed in formalin, and embedded in paraffin for immunohistochemical analysis. We examined the expression of mPGES mRNA in seven cases by reverse transcriptase-polymerase chain reaction using total RNA extracted from frozen samples. Results Immunohistochemistry revealed increased mPGES immunoreactivity in endometriosis samples compared with eutopic endometria. Microsomal PGES immunoreactivity was observed in both epithelial cells and stromal or inflammatory cells of endometriosis. Increased expression of mPGES-1 mRNA was detected in most of the endometriosis samples. Conclusion Our results suggest that expression of mPGES in addition to COX-2 plays a role in increasing PGE2 production in endometriosis. |
| Databáze: | OpenAIRE |
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