Recent Progress in Chemo-Enzymatic Methods for the Synthesis of N-Glycans
| Autor: | Meng-Hai Xiang, Xiao-Dong Gao, Yi Ding, Ning Wang, Zheng-Hui Chen, Qiang Chao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Glycan
Glycosylation 02 engineering and technology Computational biology Review N-glycosylation homogeneous glycoprotein 010402 general chemistry 01 natural sciences Chemical synthesis lcsh:Chemistry chemistry.chemical_compound N-linked glycosylation Glycosyltransferase glycosyltransferases Asparagine chemistry.chemical_classification biology Chemistry General Chemistry 021001 nanoscience & nanotechnology 0104 chemical sciences carbohydrates (lipids) lcsh:QD1-999 glycosidase biology.protein biomarker Protein folding glycosynthase 0210 nano-technology Glycoprotein chemo-enzymatic synthesis |
| Zdroj: | Frontiers in Chemistry, Vol 8 (2020) Frontiers in Chemistry |
| ISSN: | 2296-2646 |
| DOI: | 10.3389/fchem.2020.00513/full |
| Popis: | Asparagine (N)-linked glycosylation is one of the most common co- and post-translational modifications of both intra- and extracellularly distributing proteins, which directly affects their biological functions, such as protein folding, stability and intercellular traffic. Production of the structural well-defined homogeneous N-glycans contributes to comprehensive investigation of their biological roles and molecular basis. Among the various methods, chemo-enzymatic approach serves as an alternative to chemical synthesis, providing high stereoselectivity and economic efficiency. This review summarizes some recent advances in the chemo-enzymatic methods for the production of N-glycans, including the preparation of substrates and sugar donors, and the progress in the glycosyltransferases characterization which leads to the diversity of N-glycan synthesis. We discuss the bottle-neck and new opportunities in exploiting the chemo-enzymatic synthesis of N-glycans based on our research experiences. In addition, downstream applications of the constructed N-glycans, such as automation devices and homogeneous glycoproteins synthesis are also described. |
| Databáze: | OpenAIRE |
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