Effect of Curcuma zedoaria hydro-alcoholic extract on learning, memory deficits and oxidative damage of brain tissue following seizures induced by pentylenetetrazole in rat

Autor: Sedigheh Kazemi, Elham Bijad, Mahmoud Rafieian-Kopaei, Zahra Lorigooini, Touran Mahmoudi, Mehran Arabi, Zahra Rabiei
Rok vydání: 2020
Předmět:
Flumazenil
Male
0301 basic medicine
medicine.medical_treatment
Morris water navigation task
Convulsants
Pharmacology
lcsh:RC346-429
Antioxidants
Behavioral Neuroscience
Epilepsy
0302 clinical medicine
Malondialdehyde
Learning Disabilities
General Medicine
Repeated seizures
Anticonvulsants
Curcuma zedoaria
medicine.drug
Cognitive Neuroscience
Intraperitoneal injection
Nitric Oxide
03 medical and health sciences
Curcuma
food
Memory
Seizures
medicine
Animals
Rats
Wistar
Pentylenetetrazol
GABA Modulators
Maze Learning
lcsh:Neurology. Diseases of the nervous system
Biological Psychiatry
Brain Chemistry
Memory Disorders
Seizure threshold
Plant Extracts
Kindling
business.industry
Research
medicine.disease
food.food
Rats
Oxidative Stress
030104 developmental biology
Pentylenetetrazole
business
030217 neurology & neurosurgery
Zdroj: Behavioral and Brain Functions : BBF
Behavioral and Brain Functions, Vol 16, Iss 1, Pp 1-12 (2020)
ISSN: 1744-9081
DOI: 10.1186/s12993-020-00169-3
Popis: Background Previous studies have shown that seizures can cause cognitive disorders. On the other hand, the Curcuma zedoaria (CZ) has beneficial effects on the nervous system. However, there is little information on the possible effects of the CZ extract on seizures. The aim of this study was to investigate the possible effects of CZ extract on cognitive impairment and oxidative stress induced by epilepsy in rats. Methods Rats were randomly divided into different groups. In all rats (except the sham group), kindling was performed by intraperitoneal injection of pentylenetetrazol (PTZ) at a dose of 35 mg/kg every 48 h for 14 days. Positive group received 2 mg/kg diazepam + PTZ; treatment groups received 100, 200 or 400 mg/kg CZ extract + PTZ; and one group received 0.5 mg/kg flumazenil and CZ extract + PTZ. Shuttle box and Morris Water Maze tests were used to measure memory and learning. On the last day of treatments PTZ injection was at dose of 60 mg/kg, tonic seizure threshold and mortality rate were recorded in each group. After deep anesthesia, blood was drawn from the rats’ hearts and the hippocampus of all rats was removed. Results Statistical analysis of the data showed that the CZ extract significantly increased the tonic seizure threshold and reduced the pentylenetetrazol-induced mortality and the extract dose of 400 mg/kg was selected as the most effective dose compared to the other doses. It was also found that flumazenil (a GABAA receptor antagonist) reduced the tonic seizure threshold compared to the effective dose of the extract. The results of shuttle box and Morris water maze behavioral tests showed that memory and learning decreased in the negative control group and the CZ extract treatment improved memory and learning in rats. The CZ extract also increased antioxidant capacity, decreased MDA and NO in the brain and serum of pre-treated groups in compared to the negative control group. Conclusion: It is concluded that the CZ extract has beneficial effects on learning and memory impairment in PTZ-induced epilepsy model, which has been associated with antioxidant effects in the brain or possibly exerts its effects through the GABAergic system.
Databáze: OpenAIRE
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