Nasal Bone Shape Is under Complex Epistatic Genetic Control in Mouse Interspecific Recombinant Congenic Strains
| Autor: | Evelyne Heyer, Xavier Montagutelli, Michel Baylac, Gaetan Burgio |
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| Přispěvatelé: | Eco-Anthropologie et Ethnobiologie (EAE), Muséum national d'Histoire naturelle (MNHN)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Génétique Fonctionnelle de la Souris, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), University of Tasmania [Hobart, Australia] (UTAS), Origine, structure et évolution de la biodiversité (OSEB), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), The authors have no support or funding to report., We are very grateful to Isabelle Lanctin for careful breeding of the IRCS, crosses, genotyping and technical help, Marek Szatanik for genotyping and breeding help, Jean Louis Guénet for helpful discussions, Sybille Moulin for early manuscript correction, Raphael Cornette and the morphometrics platform of the Museum National d’Histoire Naturelle for skull preparation and technical help in morphometrics analysis. One anonymous reviewer is greatly acknowledged for his excellent suggestion on this manuscript, which improved its quality., Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2012 |
| Předmět: |
Male
0106 biological sciences Anatomy and Physiology Heredity Mouse MESH: Nasal Bone Gene Identification and Analysis lcsh:Medicine 01 natural sciences Genome Loss of heterozygosity Mice Molecular Cell Biology MESH: Epistasis Genetic Nasal Bone MESH: Animals lcsh:Science Musculoskeletal System Genetics 0303 health sciences Multidisciplinary Chromosome Biology Strain (biology) Chromosome Mapping Genomics Animal Models MESH: Crosses Genetic Fourier analysis Medicine Female Research Article Quantitative trait loci Linear discriminant analysis DNA recombination Mus spretus Congenic Biology Quantitative trait locus 010603 evolutionary biology Molecular Genetics Mice Congenic 03 medical and health sciences Model Organisms MESH: Mice Congenic MESH: Mice Inbred C57BL Animals Inbred strains Mammalian genomics Bone MESH: Mice Crosses Genetic Alleles 030304 developmental biology [SDV.GEN]Life Sciences [q-bio]/Genetics Quantitative Traits Skull lcsh:R Computational Biology Chromosome Epistasis Genetic biology.organism_classification MESH: Quantitative Trait Loci MESH: Male Mice Inbred C57BL Epistasis lcsh:Q Gene Function MESH: Chromosome Mapping Animal Genetics MESH: Female |
| Zdroj: | PLoS ONE, Vol 7, Iss 5, p e37721 (2012) PLoS ONE PLoS ONE, Public Library of Science, 2012, 7 (5), pp.e37721. ⟨10.1371/journal.pone.0037721⟩ PLoS ONE, 2012, 7 (5), pp.e37721. ⟨10.1371/journal.pone.0037721⟩ |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0037721 |
| Popis: | International audience; Background: Genetic determinism of cranial morphology in the mouse is still largely unknown, despite the localization of putative QTLs and the identification of genes associated with Mendelian skull malformations. To approach the dissection of this multigenic control, we have used a set of interspecific recombinant congenic strains (IRCS) produced between C57BL/6 and mice of the distant species Mus spretus (SEG/Pas). Each strain has inherited 1.3% of its genome from SEG/Pas under the form of few, small-sized, chromosomal segments.Results: The shape of the nasal bone was studied using outline analysis combined with Fourier descriptors, and differential features were identified between IRCS BcG-66H and C57BL/6. An F2 cross between BcG-66H and C57BL/6 revealed that, outof the three SEG/Pas-derived chromosomal regions present in BcG-66H, two were involved. Segments on chromosomes 1~32 Mb) and 18 (~13 Mb) showed additive effect on nasal bone shape. The three chromosomal regions present in BcG-66H were isolated in congenic strains to study their individual effect. Epistatic interactions were assessed in bicongenicstrains.Conclusions: Our results show that, besides a strong individual effect, the QTL on chromosome 1 interacts with genes onchromosomes 13 and 18. This study demonstrates that nasal bone shape is under complex genetic control but can beefficiently dissected in the mouse using appropriate genetic tools and shape descriptors. |
| Databáze: | OpenAIRE |
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