An increased frequency of numerical chromosomal abnormalities and 1p36 deletions in isolated cells from paraffin sections of malignant melanomas by means of interphase cytogenetics
| Autor: | Falko H. Herrmann, Gerd Lorenz, Manfred Pambor, Christian Woenckhaus, Thomas Dittberner, Micaela Poetsch |
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| Rok vydání: | 1998 |
| Předmět: |
Adult
Male Cancer Research medicine.medical_specialty Monosomy Numerical Chromosomal Abnormality Skin Neoplasms macromolecular substances Biology Y chromosome Genetics medicine Humans Molecular Biology Interphase Melanoma In Situ Hybridization Fluorescence Aged Paraffin Embedding medicine.diagnostic_test Hybridization probe Cytogenetics Middle Aged medicine.disease Molecular biology Chromosomes Human Pair 1 Female Chromosome Deletion Trisomy Fluorescence in situ hybridization |
| Zdroj: | Cancer genetics and cytogenetics. 104(2) |
| ISSN: | 0165-4608 |
| Popis: | At present, little information is available on tumor and stage-specific chromosomal aberrations in malignant melanoma. Therefore, we applied fluorescence in situ hybridization on isolated interphase cells from paraffin sections of 25 cases of malignant melanomas, comprising 17 primary tumors (PTs) and 8 metastases (MTs) in various anatomical sites. We used centromeric probes for chromosomes 1, 7, 9, 10, 11, 12, 15, 17, 18, X, and Y and a midisatellite probe localized in 1p36. Four of the PTs and 5 of the MTs showed polyploidy for all applied probes. The most frequent type of numerical aberration was an overrepresentation of chromosomes 1 (3 PTs, 5 MTs) and 7 (3 PTs, 1 MT), and an underrepresentation of chromosomes 9 (3 PTs) and 10 (6 PTs, 5 MTs). The Y chromosome was lost in all male tumors. In addition, we observed monosomy 11, 12, 15, 17 or 18, and trisomy 12 or 17. Only 1 PT showed no aberrations for any applied DNA probe. A deletion in the near-telomeric region of 1p36 was found surprisingly often (9 PTs, 7 MTs). Our results suggest that the loss of gene(s) in this region is an important event in the pathogenesis of malignant melanoma of the skin. |
| Databáze: | OpenAIRE |
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