Specific Functional Features of the Cell Integrity MAP Kinase Pathway in the Dimorphic Fission Yeast Schizosaccharomyces japonicus
| Autor: | José Cansado, Alejandro Franco, Jero Vicente-Soler, Beatriz Vázquez-Marín, Teresa Soto, Elisa Gómez-Gil, Marisa Madrid, Francisco Prieto-Ruiz, Armando Pérez-Díaz |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
MAPK/ERK pathway QH301-705.5 Plant Science Article 03 medical and health sciences 0302 clinical medicine hyphae Biology (General) dimorphism Ecology Evolution Behavior and Systematics Protein kinase C 030304 developmental biology 0303 health sciences biology Effector fungi S. japonicus cell integrity pathway biology.organism_classification fission yeast MAPK Yeast Cell biology S. pombe Pmk1 Mitogen-activated protein kinase Schizosaccharomyces pombe biology.protein Signal transduction 030217 neurology & neurosurgery Cytokinesis protein kinase C |
| Zdroj: | Journal of Fungi Volume 7 Issue 6 Journal of Fungi, Vol 7, Iss 482, p 482 (2021) |
| ISSN: | 2309-608X |
| DOI: | 10.3390/jof7060482 |
| Popis: | Mitogen activated protein kinase (MAPK) signaling pathways execute essential functions in eukaryotic organisms by transducing extracellular stimuli into adaptive cellular responses. In the fission yeast model Schizosaccharomyces pombe the cell integrity pathway (CIP) and its core effector, MAPK Pmk1, play a key role during regulation of cell integrity, cytokinesis, and ionic homeostasis. Schizosaccharomyces japonicus, another fission yeast species, shows remarkable differences with respect to S. pombe, including a robust yeast to hyphae dimorphism in response to environmental changes. We show that the CIP MAPK module architecture and its upstream regulators, PKC orthologs Pck1 and Pck2, are conserved in both fission yeast species. However, some of S. pombe’s CIP-related functions, such as cytokinetic control and response to glucose availability, are regulated differently in S. japonicus. Moreover, Pck1 and Pck2 antagonistically regulate S. japonicus hyphal differentiation through fine-tuning of Pmk1 activity. Chimeric MAPK-swapping experiments revealed that S. japonicus Pmk1 is fully functional in S. pombe, whereas S. pombe Pmk1 shows a limited ability to execute CIP functions and promote S. japonicus mycelial development. Our findings also suggest that a modified N-lobe domain secondary structure within S. japonicus Pmk1 has a major influence on the CIP signaling features of this evolutionarily diverged fission yeast. |
| Databáze: | OpenAIRE |
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