Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis
| Autor: | Daniel Ramírez, María Esther Rodríguez, Ismael Cross, Alberto Arias-Pérez, Manuel Alejandro Merlo, Marco Anaya, Silvia Portela-Bens, Paulino Martínez, Francisca Robles, Carmelo Ruiz-Rejón, Laureana Rebordinos |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Solea senegalensis
Chromosome evolution Comparative genomics Organic Chemistry Pleuronectiformes Karyotype General Medicine Cytogenomics Catalysis Computer Science Applications Inorganic Chemistry pleuronectiformes genetic maps cytogenomics chromosome evolution karyotype repetitive sequences comparative genomics Physical and Theoretical Chemistry Genetic maps Repetitive sequences Molecular Biology Spectroscopy |
| Zdroj: | Digibug. Repositorio Institucional de la Universidad de Granada instname International Journal of Molecular Sciences; Volume 23; Issue 10; Pages: 5353 |
| Popis: | This study was supported by the Spanish Ministry of Economy and Competitiveness, FEDER Grants (RTI2018-096847-B-C21 and RTI2018-096847-B-C22), Junta de Andalucía-FEDER Grant (P20-00938). The Pleuronectiformes order, which includes several commercially-important species, has undergone extensive chromosome evolution. One of these species is Solea senegalensis, a flatfish with 2n = 42 chromosomes. In this study, a cytogenomics approach and integration with previous maps was applied to characterize the karyotype of the species. Synteny analysis of S. senegalensis was carried out using two flatfish as a reference: Cynoglossus semilaevis and Scophthalmus maximus. Most S. senegalensis chromosomes (or chromosome arms for metacentrics and submetacentrics) showed a one-to-one macrosyntenic pattern with the other two species. In addition, we studied how repetitive sequences could have played a role in the evolution of S. senegalensis bi-armed (3, and 5–9) and acrocentric (11, 12 and 16) chromosomes, which showed the highest rearrangements compared with the reference species. A higher abundance of TEs (Transposable Elements) and other repeated elements was observed adjacent to telomeric regions on chromosomes 3, 7, 9 and 16. However, on chromosome 11, a greater abundance of DNA transposons was detected in interstitial BACs. This chromosome is syntenic with several chromosomes of the other two flatfish species, suggesting rearrangements during its evolution. A similar situation was also found on chromosome 16 (for microsatellites and low complexity sequences), but not for TEs (retroelements and DNA transposons). These differences in the distribution and abundance of repetitive elements in chromosomes that have undergone remodeling processes during the course of evolution also suggest a possible role for simple repeat sequences in rearranged regions. RTI2018-096847B-C21 AND RTI2018-096847-B-C22 SPANISH MINISTRY OF ECONOMY AND COMPETITIVENESS -FEDER |
| Databáze: | OpenAIRE |
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