GABI-DUPLO: a collection of double mutants to overcome genetic redundancy in Arabidopsis thaliana
| Autor: | Bernd Weisshaar, Nils Kleinbölting, Gunnar Huep, Dario Leister, Klaus F. X. Mayer, Georg Haberer, Cordelia Bolle |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Nuclear gene
Arabidopsis Plant Science Genome Gene Knockout Techniques Open Reading Frames Arabidopsis Thaliana Double Mutant Gene Duplication Genetic Redundancy Segmental Duplication Technical Advance Gene duplication Genetics Gene family Arabidopsis thaliana Coding region Inflorescence Gene Alleles DNA Primers biology Arabidopsis Proteins Cell Biology biology.organism_classification Plants Genetically Modified Plant Leaves Phenotype Seedlings Multigene Family Mutation Genetic redundancy Transcriptome |
| Zdroj: | Plant J. 75, 157-171 (2013) |
| Popis: | Owing to duplication events in its progenitor, more than 90% of the genes in the Arabidopsis thaliana genome are members of multigene families. A set of 2108 gene families, each consisting of precisely two unlinked paralogous genes, was identified in the nuclear genome of A. thaliana on the basis of sequence similarity. A systematic method for the creation of double knock-out lines for such gene pairs, designated as DUPLO lines, was established and 200 lines are now publicly available. Their initial phenotypic characterisation led to the identification of seven lines with defects that emerge only in the adult stage. A further six lines display seedling lethality and 23 lines were lethal before germination. Another 14 lines are known to show phenotypes under non-standard conditions or at the molecular level. Knock-out of gene pairs with very similar coding sequences or expression profiles is more likely to produce a mutant phenotype than inactivation of gene pairs with dissimilar profiles or sequences. High coding sequence similarity and highly similar expression profiles are only weakly correlated, implying that promoter and coding regions of these gene pairs display different degrees of diversification. |
| Databáze: | OpenAIRE |
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