DNA-Conjugated Gold Nanoparticles as High-Mass Probes in Imaging Mass Cytometry
| Autor: | Derek J. Wilson, Jelena Brkic, Chun Peng, Jennifer I. L. Chen, Alexandre Bouzekri, Brian Malile, Olga Ornatsky |
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| Rok vydání: | 2022 |
| Předmět: |
0303 health sciences
Biochemistry (medical) Cell Biomedical Engineering Nanoparticle 02 engineering and technology General Chemistry Conjugated system 021001 nanoscience & nanotechnology In vitro 3. Good health Biomaterials 03 medical and health sciences chemistry.chemical_compound medicine.anatomical_structure chemistry Colloidal gold medicine Biophysics High mass Mass cytometry 0210 nano-technology DNA 030304 developmental biology |
| Zdroj: | ACS applied bio materials. 2(10) |
| ISSN: | 2576-6422 |
| Popis: | We employ imaging mass cytometry (IMC) to investigate in vitro uptake and cellular distribution of DNA-functionalized gold nanoparticles (AuNPs). IMC enables the multiparametric imaging of cell components and allows for the detection of AuNPs in cells with100 times higher sensitivity than conventional confocal fluorescence imaging, as each nanoparticle contains thousands of atoms for signal amplification. Changes in the accumulation of nanoparticles in cells due to oligonucleotide sequence-dependent interactions are exploited to examine a model biomarker for hypoxia-microRNA-210. We find that AuNPs functionalized with microRNA-210-targeting sequence accumulate in hypoxic cells 3 to 4-fold compared to normoxic cells. The work examines the potential use of DNA-AuNP as high-mass probes for the analysis of nonabundant nucleic acids. |
| Databáze: | OpenAIRE |
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