Isolation and characterization of lipid-protein particles containing platelet factor 3 released from human platelets
| Autor: | H Sandberg, L O Andersson, S. Hoglund |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Blood Platelets
Phospholipid Carbohydrates Centrifugation Biochemistry Platelet Factor 3 Chromatography Affinity Sepharose chemistry.chemical_compound Membrane Lipids Affinity chromatography Phosphatidylcholine Humans Molecular Biology Phosphatidylethanolamine Chromatography Membrane Proteins Cell Biology Phosphatidylserine Phosphatidic acid Lipids Blood Coagulation Factors Microscopy Electron chemistry Chromatography Gel lipids (amino acids peptides and proteins) Electrophoresis Polyacrylamide Gel Research Article |
| Zdroj: | The Biochemical journal. 203(1) |
| ISSN: | 0264-6021 |
| Popis: | Lipid-protein particles with platelet factor 3 measured by the Stypven clotting-time test [Hardisty & Hutton (1966) Br. J. Haematol. 12, 764-776] have been isolated from platelet-release supernatant. Starting material was washed platelets, which were released by treatment with collagen. Purification of the particles from other components in the release material was accomplished by gel filtration on Sepharose CL-4B followed by affinity chromatography on poly-L-lysine-Sepharose CL-4B gel. Chemical characterization showed that the particles were composed of 40% protein, 42% phospholipids, 13% cholesterol and 5% triacylglycerols. The phospholipid composition was 38% phosphatidylcholine, 25% phosphatidylethanolamine, 9% phosphatidylserine, 2% phosphatidic acid and 26% sphingomyelin. No carbohydrate was detected. Electron-microscopic studies revealed the presence of membranous particles with diameters between 70 and 170 nm. |
| Databáze: | OpenAIRE |
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