Hypoxia Stimulates ecNOS mRNA Expression by Differentiated Human Trophoblasts
| Autor: | Susan Kadner, Thomas H. Finlay, Joseph Dancis, T. Nishiwaki, S. P. Seligman |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Gene Expression Regulation
Enzymologic General Biochemistry Genetics and Molecular Biology Andrology Syncytiotrophoblast History and Philosophy of Science Pregnancy medicine Humans RNA Messenger Cells Cultured reproductive and urinary physiology Messenger RNA Cytotrophoblast biology Chemistry General Neuroscience Trophoblast Cell Differentiation Hypoxia (medical) Cell Hypoxia Trophoblasts Nitric oxide synthase Endothelial stem cell medicine.anatomical_structure embryonic structures biology.protein Female Cytotrophoblasts Nitric Oxide Synthase medicine.symptom |
| Zdroj: | Annals of the New York Academy of Sciences. 828:180-187 |
| ISSN: | 1749-6632 0077-8923 |
| DOI: | 10.1111/j.1749-6632.1997.tb48538.x |
| Popis: | Cytotrophoblasts isolated from normal human placenta cultured under normoxic conditions (20% O2, pO2 = 130 mmHg) for 48-72 h differentiate to a form which expresses high levels of hCG and which morphologically resembles syncytiotrophoblast. We had previously shown that hypoxia (0-1% O2, pO2 = 12-14 mmHg) blocks this differentiation process, although trophoblasts exposed to hypoxia for up to 96 h were completely viable. In this article we showed that trophoblast responds to hypoxia by expressing the hypoxia-sensitive DNA binding protein HIF-1. We also showed that in trophoblast cultured under normoxic conditions, expression of endothelial cell nitric oxide synthase (ecNOS) mRNA increases with time, reaching a maximum in 48-72 h. However, in trophoblast maintained under hypoxic conditions for 48 h (after an initial 24 h in normoxia), expression of ecNOS mRNA is greatly reduced. These observations are consistent with the expression of ecNOS by syncytiotrophoblast but not by cytotrophoblast. In contrast, exposure of differentiated trophoblasts to hypoxia for 24 h (after 48-72 h in normoxia) significantly stimulates expression of ecNoS mRNA over that of cells maintained continuously in normoxia. These results suggest that in differentiated trophoblast hypoxia can stimulate ecNOS expression. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text