Engineering the iron‐oxidizing chemolithoautotroph Acidithiobacillus ferrooxidans for biochemical production
| Autor: | Xiaozheng Li, Alan C. West, Jingyang Guan, Scott Banta, Sudipta Majumdar, Timothy Kernan |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Chemoautotrophic Growth Heptadecane Acidithiobacillus Iron chemistry.chemical_element Bioengineering Applied Microbiology and Biotechnology Isobutyric acid Metabolic engineering 03 medical and health sciences chemistry.chemical_compound Isobutyrates Alkanes Oxidizing agent medicine biology Carbon Dioxide biology.organism_classification Sulfur Culture Media 030104 developmental biology Metabolic Engineering chemistry Biochemistry Biofuel Ferric Oxidation-Reduction Bacteria Biotechnology medicine.drug |
| Zdroj: | Biotechnology and Bioengineering. 113:189-197 |
| ISSN: | 1097-0290 0006-3592 |
| Popis: | There is growing interest in developing non-photosynthetic routes for the conversion of CO2 to fuels and chemicals. One underexplored approach is the transfer of energy to the metabolism of genetically modified chemolithoautotrophic bacteria. Acidithiobacillus ferrooxidans is an obligate chemolithoautotroph that derives its metabolic energy from the oxidation of iron or sulfur at low pH. Two heterologous biosynthetic pathways have been expressed in A. ferrooxidans to produce either isobutyric acid or heptadecane from CO2 and the oxidation of Fe(2+). A sevenfold improvement in productivity of isobutyric acid was obtained through improved media formulations in batch cultures. Steady-state efficiencies were lower in continuous cultures, likely due to ferric inhibition. If coupled to solar panels, the photon-to-fuel efficiency of this proof-of-principle process approaches estimates for agriculture-derived biofuels. These efforts lay the foundation for the utilization of this organism in the exploitation of electrical energy for biochemical synthesis. |
| Databáze: | OpenAIRE |
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