Pet Pyometra: Correlating Bacteria Pathogenicity to Endometrial Histological Changes

Autor: Franciele Maboni Siqueira, Camila Imperico Riboldi, Cassiane Elisabete Lopes, Welden Panziera, David Driemeier, Cíntia de Lorenzo, Silvia De Carli
Rok vydání: 2021
Předmět:
0301 basic medicine
Microbiology (medical)
medicine.medical_specialty
Microbiological culture
Cães
040301 veterinary sciences
Uterus
Histopathology
Virulence
Piometra
Biology
medicine.disease_cause
Endometrium
Article
Análise bacteriológica
Microbiology
0403 veterinary science
03 medical and health sciences
Doencas uterinas
uterus infection
companion animals
Escherichia coli
medicine
Immunology and Allergy
bacteria isolation
Molecular Biology
Fatores de virulência
General Immunology and Microbiology
virulence genes
04 agricultural and veterinary sciences
Companion animals
Pyometra
medicine.disease
biology.organism_classification
Histopatologia
Uterus infection
Gatos
EnPEC
030104 developmental biology
Infectious Diseases
medicine.anatomical_structure
Virulence genes
Bacteria isolation
histopathology
Medicine
Bacteria
Zdroj: Repositório Institucional da UFRGS
Universidade Federal do Rio Grande do Sul (UFRGS)
instacron:UFRGS
Pathogens
Pathogens, Vol 10, Iss 833, p 833 (2021)
Volume 10
Issue 7
ISSN: 2076-0817
DOI: 10.3390/pathogens10070833
Popis: Pyometra is a life-threatening infectious disease that frequently affects bitches and queens. Although histopathological patterns of pyometra have been extensively explored, the microbiological aspects, such as bacteria pathogenicity, have not been correlated to microscopy endometrial lesions so far. In this study, these two pathological aspects of pyometra were analysed and correlated. Uterus fragments and intrauterine content samples were collected from pets diagnosed with pyometra (30) and submitted to histopathology analysis and bacterial culture, respectively. The degree of endometrial histopathological lesions in pyometra cases were classified as mild, moderate and severe. Thirty different bacteria isolates were identified from intrauterine content culture. Escherichia coli (E. coli) was pure isolated in 57.7% and highly related to severe endometrial lesions. Immunohistochemistry assay revealed the adhesion and invasion of this bacteria agent to the injured endometrium. Virulence aspects of these E. coli strains were explored, demonstrating biofilm formation ability and a set of virulence genes in most isolates. These results support the adaptive genetic and phenotypic advantages of E. coli for uterus infection, and justify the high frequency of this agent involved in pyometra cases.
Databáze: OpenAIRE
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