| Popis: |
Additional file 2: Figure S2. Erythroid-selective accessibility of the β-globin cluster. ATAC-seq signal tracks of the six cell types in this study at the β-globin cluster (chr7: 103,792,027–103,879,340; mm10). The adult globin genes β-major (ßmaj) and β-minor (ßmin), as well as the hypersensitive sites (HS1-4,6) of the Locus Control Region (LCR) that regulates expression of the genes in this locus, displayed accessibility in EPs. HS2, but not the other HSs, and the β-major promoter were also accessible in HSCs, possibly indicating a “permissive” chromatin state. Accessibility of HS2 and HS4 in MkPs may relate to a closer relationship to HSCs and/or EPs (Fig. 1). As expected, no accessibility was observed at the fetal-specific epsilon Y globin (Ey), β-h1 (ßh1), β-h2 (ßh2) genes, or HS5. Likewise, GMs, B and T cells, that do not express β-globin genes, did not display accessibility of any of the regulatory elements in the locus. |
