Diagnostic efficacy of monoclonal antibody based sandwich enzyme linked immunosorbent assay (ELISA) for detection of Fasciola gigantica excretory/secretory antigens in both serum and stool
| Autor: | Zeinab Demerdash, Mohy El-Deen Attia, Wafaa Mansour, Ibrahim Aly, Tarek M. Diab, Mona Zoheiry, Azza E I El-Bassiouny, Salwa H. Mohamed, Faten S. Mahmoud |
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| Rok vydání: | 2011 |
| Předmět: |
Serum
Fascioliasis Seroantigen medicine.drug_class Fasciola gigantica Enzyme-Linked Immunosorbent Assay Coproantigen Monoclonal antibody Sensitivity and Specificity Immunoglobulin G lcsh:Infectious and parasitic diseases Feces Mice Antigen medicine Animals Humans lcsh:RC109-216 chemistry.chemical_classification Mice Inbred BALB C biology Clinical Laboratory Techniques Research Antibodies Monoclonal biology.organism_classification Virology Molecular biology Infectious Diseases Enzyme Sandwich ELISA Parasitology chemistry Antigens Helminth biology.protein Monoclonal antibodies Antibody |
| Zdroj: | Parasites & Vectors, Vol 4, Iss 1, p 176 (2011) Parasites & Vectors |
| ISSN: | 1756-3305 |
| Popis: | Background This research was carried out to develop a reliable monoclonal antibody (MoAb)-based sandwich enzyme linked immunosorbent assay (ELISA) for the diagnosis of active Fasciola gigantica infection in both serum and stool for comparative purposes. Methods From a panel of MoAbs raised against F. gigantica excretory/secretory antigens (ES Ags), a pair (12B/11D/3F and 10A/9D/10G) was chosen due to its high reactivity and strict specificity to F. gigantica antigen by indirect ELISA. Results The two MoAbs were of the IgG1 and IgG2a subclasses, respectively. Using SDS-PAGE and EITB, the selected MoAbs recognized 83, 64, 45 and 26 kDa bands of ES Ags. The lower detection limit of ELISA assay was 3 ng/ml. In stool, the sensitivity, specificity and diagnostic efficacy of ELISA was 96%, 98.2 and 97.1%; while in serum they were 94%, 94.6% and 94.3%, respectively. Moreover, a positive correlation was found between ova count in stool of F. gigantica infected patients and the OD readings of ELISA in both stool and serum samples (r = 0.730, p < 0.01 and r = 0.608; p < 0.01, respectively). Conclusions These data showed that the use of MoAb-based sandwich ELISA for the detection of F. gigantica coproantigens in stool specimens was superior to serum samples; it provides a highly efficient, non-invasive technique for the diagnosis of active F. gigantica infection. |
| Databáze: | OpenAIRE |
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