GlcNAc6ST3 is a keratan sulfate sulfotransferase for the protein-tyrosine phosphatase PTPRZ in the adult brain
| Autor: | Kenji Uchimura, Narentuya, Yoshiko Takeda-Uchimura, Tomoya O. Akama, Kenji Kadomatsu, Zui Zhang, Tahmina Foyez, Yukio Komatsu, Hirokazu Yagi, Koichi Kato |
|---|---|
| Přispěvatelé: | Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université de Lille, CNRS, Nagoya University, Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF] |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Gene isoform Male Sulfotransferase Keratan sulfate [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology Phosphatase Synaptophysin lcsh:Medicine Protein tyrosine phosphatase Article 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Animals Immunoprecipitation [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology lcsh:Science Multidisciplinary Microscopy Confocal biology Chemistry Receptor-Like Protein Tyrosine Phosphatases Class 5 Perineuronal net lcsh:R Brain [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Cell biology 030104 developmental biology Proteoglycan Keratan Sulfate PTPRZ1 biology.protein lcsh:Q [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] Female Proteoglycans Protein Tyrosine Phosphatases Sulfotransferases 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports Scientific Reports, Nature Publishing Group, 2019, 9 (1), ⟨10.1038/s41598-019-40901-2⟩ Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019) Scientific Reports, 2019, 9 (1), ⟨10.1038/s41598-019-40901-2⟩ |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-40901-2⟩ |
| Popis: | Keratan sulfate (KS) is a carbohydrate side chain covalently attached to extracellular proteoglycans. KS is composed of disaccharide units of 6-sulfated N-acetylglucosamine (GlcNAc) and galactose. We have previously shown that GlcNAc-6-O-sulfotransferase (GlcNAc6ST) 1 encoded by Chst2 is an enzyme necessary for the synthesis of GlcNAc-6-sulfated KS chains that are required for neuronal plasticity in the visual cortex of the mouse brain during the critical period, but not in adulthood. Here, we show that GlcNAc-6-sulfated KS recognized by the R-10G anti-KS antibody, of which the minimum epitope structure is Galß1-4GlcNAc(6S)ß1-3Galß1-4GlcNAc(6S), distributes diffusely in neuropils and presents densely in close proximity to the perineuronal region of the perineuronal net (PNN)-positive neurons in the adult visual cortex. Surprisingly, GlcNAc6ST3, which was discovered as an intestinal GlcNAc6ST encoded by Chst5, is a major brain KS sulfotransferase expressed in oligodendrocytes in adulthood. Moreover, we identified an isoform of the protein-tyrosine phosphatase PTPRZ as a R-10G-reactive KS proteoglycan. These results indicate that GlcNAc6ST3 may play a role in synthesis of a component of PNN in the adult brain, and that the KS-modified isoform of PTPRZ encoded by Ptprz1 could be an extracellular molecule associated with PNNs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|