Non-cell-autonomous migration of RasV12-transformed cells towards the basal side of surrounding normal cells
| Autor: | Toshiki Itoh, Imen Jebri, Yasuyuki Fujita, Kazuya Tsujita |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Basal extrusion NRK-52E Biophysics Motility Oncogene Protein p21(ras) Biochemistry Phosphatidylinositol 3-Kinases 03 medical and health sciences Basal (phylogenetics) Dogs 0302 clinical medicine Cell Movement Neoplasms Cancer cell invasion medicine Animals Humans Amino Acid Sequence Molecular Biology Cells Cultured PI3K/AKT/mTOR pathway Myosin II Gene knockdown Chemistry Nonmuscle Myosin Type IIA Cancer Valine Cell Biology medicine.disease Epithelium Rats Cell biology Transformation (genetics) 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Mutation Cancer cell Signal Transduction Ras |
| Zdroj: | Biochemical and Biophysical Research Communications. 543:15-22 |
| ISSN: | 0006-291X |
| Popis: | Oncogenic transformation enables cells to behave differently from their neighboring normal cells. Both cancer and normal cells recognize each other, often promoting the extrusion of the former from the epithelial cell layer. Here, we show that RasV12-transformed normal rat kidney 52E (NRK-52E) cells are extruded towards the basal side of the surrounding normal cells, which is concomitant with enhanced motility. The active migration of the basally extruded RasV12 cells is observed when surrounded by normal cells, indicating a non-cell-autonomous mechanism. Furthermore, specific inhibitor treatment and knockdown experiments elucidate the roles of PI3K and myosin IIA in the basal extrusion of Ras cells. Our findings reveal a new aspect of cancer cell invasion mediated by functional interactions with surrounding non-transformed cells. |
| Databáze: | OpenAIRE |
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