Survival Rate of Cells Sent by a Low Mechanical Load Tube Pump: The 'Ring Pump'
| Autor: | Inoue Hiroaki, Kaoru Uesugi, Yoji Yamada, Yoichi Sakurai, Keisuke Morishima, Koki Hirai, Keizo Nishiyama, Takashi Taniguchi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Materials science
lcsh:Mechanical engineering and machinery cell culture system Peristaltic pump Ring (chemistry) 01 natural sciences Article 03 medical and health sciences stomatognathic system Tube (fluid conveyance) lcsh:TJ1-1570 Electrical and Electronic Engineering Survival rate Cell survival 030304 developmental biology ring pump microfluidic system 0303 health sciences Mechanical load Mechanical Engineering mid-press ring pump 010401 analytical chemistry 0104 chemical sciences full-press ring pump Control and Systems Engineering Cell culture Large size Biomedical engineering |
| Zdroj: | Micromachines Volume 11 Issue 4 Micromachines, Vol 11, Iss 447, p 447 (2020) |
| ISSN: | 2072-666X |
| DOI: | 10.3390/mi11040447 |
| Popis: | A ring pump (RP) is a useful tool for microchannels and automated cell culturing. We have been developing RPs (a full-press ring pump, FRP and a mid-press ring pump, MRP). However, damage to cells which were sent by the RP and the MRP was not investigated, and no other studies have compared the damage to cells between RPs and peristaltic pumps (PPs). Therefore, first, we evaluated the damage to cells that were sent by a small size FRP (s-FRP) and small size MRPs (s-MRPs gap = 25 or 50 &mu m, respectively). &ldquo Small size&rdquo means that the s-FRP and the s-MRPs are suitable for microchannel-scale applications. The survival rate of cells sent by the s-MRPs was higher than those sent by the s-FRP, and less damage caused by the former. Second, we compared the survival rate of cells that were sent by a large size FRP (l-FRP), a large size MRP (l-MRP) (gap = 50 &mu m) and a PP. &ldquo Large size&rdquo means that the l-FRP and the l-MRP are suitable for automated cell culture system applications. We could not confirm any differences among the cell survival rates. On the other hand, when cells suspended in Dulbecco&rsquo s phosphate-buffered saline solution were circulated with the l-MRP (gap = 50 &mu m) and the PP, we confirmed a difference in cell survival rate, and less damage caused by the former. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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