Cyclooxygenase-1 and 2 in normal and malignant human ovarian epithelium
Autor: | Shuanfang Li, J. Carl Barrett, Barbara J. Davis, Rick D. Fannin, Kelly Miner |
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Rok vydání: | 2003 |
Předmět: |
Adult
Pathology medicine.medical_specialty Immunoblotting Epithelium Western blot Cell Line Tumor medicine Humans Neoplasm Metastasis Neoplasm Staging Ovarian Neoplasms medicine.diagnostic_test business.industry Ovary Obstetrics and Gynecology Cancer Membrane Proteins Middle Aged medicine.disease Isoenzymes medicine.anatomical_structure Oncology Membrane protein Cell culture Cyclooxygenase 2 Prostaglandin-Endoperoxide Synthases Cyclooxygenase 1 Immunohistochemistry Female business Ovarian cancer Immunostaining |
Zdroj: | Gynecologic oncology. 92(2) |
ISSN: | 0090-8258 |
Popis: | Objectives . Cyclooxygenase-1 and 2 (COX-1 and COX-2) play important roles in normal physiology and are often dysregulated in neoplastic tissues. The present study determines whether COX-1 and COX-2 are expressed in ovarian cancers and whether the pattern of expression of these enzymes reveals clues to their roles in this cancer. Methods . The expression of COX-1 and COX-2 proteins in 9 normal human ovaries, in 137 cases of ovarian cancers of epithelial origin (83 primary and 54 metastatic), and in 7 ovarian cancer cell lines was examined by immunohistochemistry and western analysis. Results . COX-1 protein was present in 95/137 (69.3%) of the total cancers studied, with 55/83 (66.3%) of the primary cancers and 40/54 (74.1%) of the metastatic cancers positive for protein. COX-2 was present in 97/137 (70.8%) of all cancers studied, with 53/83 (63.9%) of the primary cancers and 44/54 (81.5%) of the metastatic cancers positive for protein. Notably, the quickscores for COX-2-positive staining were significantly higher in metastatic cancers. Moreover, COX-2 immunostaining was frequently found at the advancing margin of tumor invasion or in new metastatic loci. COX-1 protein expression was observed in the ovarian surface epithelial cells, especially that of the inclusion cysts. COX-1 was also detected by western blot in seven of nine ovarian cancer cell lines. However, no COX-2 was detected in either normal epithelium or cancer cell lines. Conclusion . COX-1 and COX-2 were expressed in every type of ovarian epithelial cancer, suggesting that each may contribute to the cancer development or progression. |
Databáze: | OpenAIRE |
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