Elevated Plasma Von Willebrand Factor and Propeptide Levels in Malawian Children with Malaria
| Autor: | Bridon M'baya, E. Brian Faragher, Karl B. Seydel, Malcolm E. Molyneux, Terrie E. Taylor, Jan A. van Mourik, Alister Craig, James Bunn, Simon J. Glover, Daniel J. Bridges, Bas de Laat, Happy T. Phiri |
|---|---|
| Přispěvatelé: | Biochemie, RS: CARIM School for Cardiovascular Diseases |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Male
Malawi wh_322 lcsh:Medicine Pathogenesis Biochemistry Pediatrics 0302 clinical medicine Blood plasma Pathology Platelet Prospective Studies Young adult Malaria Falciparum Prospective cohort study Child lcsh:Science Neuropathology 0303 health sciences education.field_of_study Multidisciplinary biology Child Health wh_20 ws_20 Hematology 3. Good health Plasmodium Falciparum Infectious Diseases Neurology Cerebral Malaria Child Preschool Coagulation disorders Medicine Retinal Disorders Female Public Health von Willebrand disease ws_100 Research Article Adult wh_100 Adolescent Fever 030231 tropical medicine Population Malaria Cerebral Enzyme-Linked Immunosorbent Assay Sensitivity and Specificity Microbiology Diagnosis Differential 03 medical and health sciences Young Adult Von Willebrand factor Retinal Diseases Diagnostic Medicine von Willebrand Factor parasitic diseases medicine Parasitic Diseases Humans Pediatric Hematology Protein Precursors education Biology 030304 developmental biology Plasma Proteins business.industry lcsh:R Infant Proteins Tropical Diseases (Non-Neglected) medicine.disease Malaria Ophthalmoscopy Ophthalmology Anatomical Pathology Immunology biology.protein lcsh:Q business Biomarkers General Pathology |
| Zdroj: | PLoS ONE, Vol 6, Iss 11, p e25626 (2011) PLoS ONE PLOS ONE, 6(11):e25626. Public Library of Science |
| ISSN: | 1932-6203 |
| Popis: | Background In spite of the significant mortality associated with Plasmodium falciparum infection, the mechanisms underlying severe disease remain poorly understood. We have previously shown evidence of endothelial activation in Ghanaian children with malaria, indicated by elevated plasma levels of both von Willebrand factor (VWF) and its propeptide. In the current prospective study of children in Malawi with retinopathy confirmed cerebral malaria, we compared these markers with uncomplicated malaria, non malarial febrile illness and controls. Methods and Findings Children with cerebral malaria, mild malaria and controls without malaria were recruited into the study. All comatose patients were examined by direct and indirect ophthalmoscopy. Plasma VWF and propeptide levels were measured by ELISA. Median VWF and propeptide levels were significantly higher in patients with uncomplicated malaria than in children with non-malarial febrile illness of comparable severity, in whom levels were higher than in non-febrile controls. Median concentrations of both markers were higher in cerebral malaria than in uncomplicated malaria, and were similar in patients with and without retinopathy. Levels of both VWF and propeptide fell significantly 48 hours after commencing therapy and were normal one month later. Conclusions In children with malaria plasma VWF and propeptide levels are markedly elevated in both cerebral and mild paediatric malaria, with levels matching disease severity, and these normalize upon recovery. High levels of both markers also occur in retinopathy-negative ‘cerebral malaria’ cases, many of whom are thought to be suffering from diseases other than malaria, indicating that further studies of these markers will be required to determine their sensitivity and specificity. |
| Databáze: | OpenAIRE |
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