Instillation versus Inhalation of Multiwalled Carbon Nanotubes: Exposure-Related Health Effects, Clearance, and the Role of Particle Characteristics
| Autor: | Somenath Mitra, Paul Westerhoff, Laura S. Van Winkle, Lisa M. Franzi, Zheqiong Wu, Donald S. Anderson, James E. Evans, Otto G. Raabe, Gavin Dutrow, Rona M. Silva, Christel TeeSy, Kent E. Pinkerton, Vincent Vu, Kyle Doudrick |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Materials science Chemical Phenomena Neutrophils Pulmonary toxicity Carboxylic Acids General Physics and Astronomy Inflammation Pharmacology Article Rats Sprague-Dawley Toxicology Administration Inhalation Toxicity Tests medicine Animals General Materials Science multiwalled carbon nanotube (MWCNT) pulmonary toxicity Inhalation exposure Lung Dose-Response Relationship Drug medicine.diagnostic_test Inhalation Nanotubes Carbon Macrophages General Engineering Water Cell Differentiation respiratory system Neutrophilia Rats respiratory tract diseases 3. Good health inhalation exposure engineered nanomaterial Instillation Drug Bronchoalveolar lavage medicine.anatomical_structure Health inflammation Toxicity medicine.symptom Bronchoalveolar Lavage Fluid |
| Zdroj: | ACS Nano |
| ISSN: | 1936-086X 1936-0851 |
| DOI: | 10.1021/nn503887r |
| Popis: | Inhaled multiwalled carbon nanotubes (MWCNTs) may cause adverse pulmonary responses due to their nanoscale, fibrous morphology and/or biopersistance. This study tested multiple factors (dose, time, physicochemical characteristics, and administration method) shown to affect MWCNT toxicity with the hypothesis that these factors will influence significantly different responses upon MWCNT exposure. The study is unique in that (1) multiple administration methods were tested using particles from the same stock; (2) bulk MWCNT formulations had few differences (metal content, surface area/functionalization); and (3) MWCNT retention was quantified using a specialized approach for measuring unlabeled MWCNTs in rodent lungs. Male Sprague–Dawley rats were exposed to original (O), purified (P), and carboxylic acid functionalized (F) MWCNTs via intratracheal instillation and inhalation. Blood, bronchoalveolar lavage fluid (BALF), and lung tissues were collected at postexposure days 1 and 21 for quantifying biological responses and MWCNTs in lung tissues by programmed thermal analysis. At day 1, MWCNT instillation produced significant BALF neutrophilia and MWCNT-positive macrophages. Instilled O- and P-MWCNTs produced significant inflammation in lung tissues, which resolved by day 21 despite MWCNT retention. MWCNT inhalation produced no BALF neutrophilia and no significant histopathology past day 1. However, on days 1 and 21 postinhalation of nebulized MWCNTs, significantly increased numbers of MWCNT-positive macrophages were observed in BALF. Results suggest (1) MWCNTs produce transient inflammation if any despite persistence in the lungs; (2) instilled O-MWCNTs cause more inflammation than P- or F-MWCNTs; and (3) MWCNT suspension media produce strikingly different effects on physicochemical particle characteristics and pulmonary responses. |
| Databáze: | OpenAIRE |
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