Determining intracellular lipid content of different oleaginous yeasts by one simple and accurate Nile Red fluorescent method
| Autor: | Lian Xiong, Chao Huang, Cheng Zhao, Chen Xuefang, Xinde Chen, Hailong Li, Mu-Tan Luo |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
01 natural sciences Biochemistry chemistry.chemical_compound Trichosporon Yeasts 010608 biotechnology Oxazines Lipomyces Fluorescent Dyes Microscopy Confocal Chromatography Staining and Labeling 010405 organic chemistry Chemistry Temperature Nile red food and beverages General Medicine Lipids Fluorescence 0104 chemical sciences Staining Standard curve Cryptococcus Lipid content Intracellular Biotechnology |
| Zdroj: | Preparative Biochemistry and Biotechnology. 49:597-605 |
| ISSN: | 1532-2297 1082-6068 |
| Popis: | A simple and accurate Nile Red fluorescent method was built to evaluate the lipid content of three different oleaginous yeasts by one standard curve. The staining of cells can be observed clearly by laser scanning confocal microscope, showing that Nile Red can enter into the cells of oleaginous yeasts easily. A series of conditions such as pretreating temperature, cell suspension concentration (OD |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|