Identification and expression analysis of duck interleukin-17D in Riemerella anatipestifer infection
| Autor: | Suk Kim, Hyun S. Lillehoj, Sungwon Kim, Joyce Anne R. Diaz, Rami A. Dalloul, Fahmida Afrin, Wongi Min, Cherry P. Fernandez, Woo H. Kim, Jipseol Jeong |
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| Přispěvatelé: | Animal and Poultry Sciences |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Interleukin-27 Immunology Spleen Biology Riemerella Proinflammatory cytokine Microbiology Avian Proteins 03 medical and health sciences Interleukin-17D 0302 clinical medicine Immune system Flavobacteriaceae Infections medicine Avian IL-17D Animals Lymphocytes Transgenes Cloning Molecular Interleukin 27 Riemerella infection Phylogeny Regulation of gene expression Gene Expression Profiling Interleukin Riemerella anatipestifer Biological Evolution Virology Immunity Innate Ducks 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Cytokines Inflammation Mediators 030215 immunology Developmental Biology |
| Zdroj: | Developmental & Comparative Immunology. 61:190-197 |
| ISSN: | 0145-305X |
| DOI: | 10.1016/j.dci.2016.04.002 |
| Popis: | Interleukin (IL)-17D is a proinfiammatory cytokine with currently largely unknown biological functions. Here we provide the description of the sequence, bioactivity, and mRNA expression profile of duck IL-17D homologue. A full-length duck IL-17D (duIL-17D) cDNA with a 624-bp coding region was identified from the large intestine. dull-17D shares approximately 94.7% identity with its chicken counterpart, which is also identified in this work. duIL-17D exhibits 62.6-68.4% and 52.1-53.1% identity with mammalian and piscine homologues. Recombinant duIL-17D promoted the expression of proinfiammatory cytokines such as IL-6, IL-8, and IL-1 beta in duck embryo fibroblast cells. Very low levels of dull-17D transcript were observed in healthy lymphoid tissues, including bursa, thymus, and spleen, while duIL-17D expression was relatively high in the heart. The dull-17D expression profiles were examined in mitogen-stimulated splenic lymphocytes, as well as tissues affected by Riemerella anatipestifer infection. The levels of duIL-17D were mostly upregulated in mitogen-activated splenic lymphocytes but downregulated in the liver and spleen of R. anatipestifer-infected ducks. These results provide new insights into the roles of IL-17D in host protective immune responses to Riemerella infection, which can therefore lead to further studies of its biological functions in different disease models of ducks and other avian species. Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2015R1D1A1A02059953] This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2015R1D1A1A02059953). Public domain – authored by a U.S. government employee |
| Databáze: | OpenAIRE |
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