The Proto-Oncogene c- myc Is a Direct Target Gene of Epstein-Barr Virus Nuclear Antigen 2
| Autor: | Nicola Maria Jochner, Georg W. Bornkamm, Carmen Kaiser, Gerhard Laux, Dirk Eick, Bettina Kempkes |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
viruses
Immunology Biology Proto-Oncogene Mas Microbiology Virus Proto-Oncogene Proteins c-myc Viral Matrix Proteins Cyclin D2 Cyclin-dependent kinase Cyclins Proto-Oncogene Proteins hemic and lymphatic diseases Virology Humans Cell Line Transformed Cyclin-dependent kinase 4 Cyclin-Dependent Kinase 4 Cell cycle Cyclin-Dependent Kinases Virus-Cell Interactions Cell biology Gene Expression Regulation Neoplastic Epstein-Barr Virus Nuclear Antigens Cell culture Insect Science biology.protein Epstein–Barr virus nuclear antigen 2 Immortalised cell line |
| Zdroj: | Journal of Virology. 73:4481-4484 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.73.5.4481-4484.1999 |
| Popis: | Epstein-Barr virus (EBV) infects and transforms primary B lymphocytes in vitro. Viral infection initiates the cell cycle entry of the resting B lymphocytes. The maintenance of proliferation in the infected cells is strictly dependent on functional EBNA2. We have recently developed a conditional immortalization system for EBV by rendering the function of EBNA2, and thus proliferation of the immortalized cells, dependent on estrogen. This cellular system was used to identify early events preceding induction of proliferation. We show that LMP1 and c- myc are directly activated by EBNA2, indicating that all cellular factors essential for induction of these genes by EBNA2 are present in the resting cells. In contrast, induction of the cell cycle regulators cyclin D2 and cdk4 are secondary events, which require de novo protein synthesis. |
| Databáze: | OpenAIRE |
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