Clinorotation-induced weightlessness influences the cytoskeleton of glial cells in culture
| Autor: | Paola Prato, Bianca Maria Uva, Felice Strollo, Maddalena Sturla, Massimo Giuliani, Grazia Tagliafierro, Mario Passalacqua, Maria Angela Masini |
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| Rok vydání: | 2002 |
| Předmět: |
Central Nervous System
Rotation Intermediate Filaments Fluorescent Antibody Technique Mitosis Vimentin Apoptosis macromolecular substances Microtubules chemistry.chemical_compound Tubulin Glial Fibrillary Acidic Protein medicine Tumor Cells Cultured Animals Propidium iodide Intermediate filament Cytoskeleton Molecular Biology Weightlessness Simulation Cell Size Cell Nucleus Microscopy Confocal biology Random positioning machine Glial fibrillary acidic protein Weightlessness General Neuroscience Actins Cell biology Rats medicine.anatomical_structure chemistry biology.protein Microscopy Electron Scanning Neuroglia Neurology (clinical) Space Motion Sickness Clinostat Developmental Biology |
| Zdroj: | Brain research. 934(2) |
| ISSN: | 0006-8993 |
| Popis: | During and after spaceflight astronauts experience neurophysiological alterations. To investigate if the impairment observed might be traced back to cytomorphology, we undertook a ground based research using a random positioning machine (clinostat) as a simulation method for microgravity. The outcome of the study was represented by cytoskeletal changes occurring in cultured glial cells (C(6) line) after 15 min, 30 min, 1 h, 20 h and 32 h under simulated microgravity. Glia is fundamental for brain function and it is essential for the normal health of the entire nervous system. Our data showed that after 30 min under simulated microgravity the cytoskeleton was damaged: microfilaments (F-actin) and intermediate filaments (Vimentin, Glial Fibrillary Acidic Proteins GFAP) were highly disorganised, microtubules (alpha-tubulin) lost their radial array, the overall cellular shape was deteriorated, and the nuclei showed altered chromatin condensations and DNA fragmentation. This feature got less dramatic after 20 h of simulated microgravity when glial cells appeared to reorganise their cytoskeleton and mitotic figures were present. The research was carried out by immunohistochemistry using antibodies to alpha-tubulin, vimentin and GFAP, and cytochemical labelling of F-actin (Phalloidin-TRIC). The nuclei were stained with propidium iodide or 4,6-diamidino-2-phenylindole dihydrochloride (DAPI). The cells were observed at the conventional and/or the confocal laser scanning microscope. Samples were also observed at the scanning electron microscope (SEM). Our data showed that in weightlessness alterations occur already visible at the scale of the single cell; if this may lead to the neurophysiological problems observed in flight is yet to be established. |
| Databáze: | OpenAIRE |
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