Assessing the prognostic value of tumor-infiltrating CD57+ cells in advanced stage head and neck cancer using QuPath digital image analysis

Autor: Emma J. de Ruiter, Sangeeta K. Bisheshar, Reinout H. de Roest, Frederik W. R. Wesseling, Frank J. P. Hoebers, Mari F. C. M. van den Hout, C. René Leemans, Ruud H. Brakenhoff, Remco de Bree, Chris H. J. Terhaard, Stefan M. Willems
Přispěvatelé: Radiotherapie, RS: GROW - R3 - Innovative Cancer Diagnostics & Therapy, MUMC+: DA Pat Pathologie (9), RS: GROW - R2 - Basic and Translational Cancer Biology, Damage and Repair in Cancer Development and Cancer Treatment (DARE), Guided Treatment in Optimal Selected Cancer Patients (GUTS), Otolaryngology / Head & Neck Surgery, CCA - Cancer biology and immunology, CCA - Imaging and biomarkers, General practice
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Virchows Archiv, 481(2), 223-231. Springer, Cham
Virchows Archiv, 481(2), 223-231. SPRINGER
Virchows Archiv, 481(2), 223-231. Springer Verlag
de Ruiter, E J, Bisheshar, S K, de Roest, R H, Wesseling, F W R, Hoebers, F J P, van den Hout, M F C M, Leemans, C R, Brakenhoff, R H, de Bree, R, Terhaard, C H J & Willems, S M 2022, ' Assessing the prognostic value of tumor-infiltrating CD57+ cells in advanced stage head and neck cancer using QuPath digital image analysis ', Virchows Archiv, vol. 481, no. 2, pp. 223-231 . https://doi.org/10.1007/s00428-022-03323-6
ISSN: 0945-6317
DOI: 10.1007/s00428-022-03323-6
Popis: This study aimed to assess the prognostic value of intratumoral CD57+ cells in head and neck squamous cell carcinoma (HNSCC) and to examine the reproducibility of these analyses using QuPath. Pretreatment biopsies of 159 patients with HPV-negative, stage III/IV HNSCC treated with chemoradiotherapy were immunohistochemically stained for CD57. The number of CD57+ cells per mm2 tumor epithelium was quantified by two independent observers and by QuPath, software for digital pathology image analysis. Concordance between the observers and QuPath was assessed by intraclass correlation coefficients (ICC). The correlation between CD57 and clinicopathological characteristics was assessed; associations with clinical outcome were estimated using Cox proportional hazard analysis and visualized using Kaplan-Meier curves. The patient cohort had a 3-year OS of 65.8% with a median follow-up of 54 months. The number of CD57+ cells/mm2 tumor tissue did not correlate to OS, DFS, or LRC. N stage predicted prognosis (OS: HR 0.43, p = 0.008; DFS: HR 0.41, p = 0.003; LRC: HR 0.24, p = 0.007), as did WHO performance state (OS: HR 0.48, p = 0.028; LRC: 0.33, p = 0.039). Quantification by QuPath showed moderate to good concordance with two human observers (ICCs 0.836, CI 0.805–0.863, and 0.741, CI 0.692–0.783, respectively). In conclusion, the presence of CD57+ TILs did not correlate to prognosis in advanced stage, HPV-negative HNSCC patients treated with chemoradiotherapy. Substantial concordance between human observers and QuPath was found, confirming a promising future role for digital, algorithm driven image analysis.
Databáze: OpenAIRE
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