Anti-hyperglycemic activity of myricetin, through inhibition of DPP-4 and enhanced GLP-1 levels, is attenuated by co-ingestion with lectin-rich protein

Autor: Bettadahalli Sadashivaiah, Nanjaiah Lalitha, Sridevi Annapurna Singh, Talahalli Ravichandra Ramaprasad
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Blood Glucose
Physiology
Inflammasomes
medicine.medical_treatment
Gene Expression
Pharmacology
Biochemistry
Antioxidants
chemistry.chemical_compound
Endocrinology
Glucagon-Like Peptide 1
Pregnancy
Medicine and Health Sciences
Insulin
Plant Proteins
Multidisciplinary
Immune System Proteins
Chemistry
Proteolytic enzymes
myr
Inflammasome
Fabaceae
Blood Sugar
Body Fluids
Blood
Liver
Medicine
Myricetin
Female
Anatomy
medicine.drug
Research Article
animal structures
Endocrine Disorders
Dipeptidyl Peptidase 4
Science
Immunology
Blood Plasma
Diabetes Mellitus
Experimental
03 medical and health sciences
Diabetes mellitus
NLR Family
Pyrin Domain-Containing 3 Protein
medicine
Genetics
Diabetes Mellitus
Animals
Hypoglycemic Agents
Rats
Wistar
Dipeptidyl peptidase-4
Diabetic Endocrinology
Flavonoids
030109 nutrition & dietetics
Biology and Life Sciences
Proteins
Streptozotocin
medicine.disease
Hormones
Gastrointestinal Tract
Oxidative Stress
030104 developmental biology
Metabolic Disorders
Digestive System
Zdroj: PLoS ONE, Vol 15, Iss 4, p e0231543 (2020)
PLoS ONE
ISSN: 1932-6203
Popis: Dipeptidyl peptidase-4 (DPP-4) is a proteolytic enzyme responsible for the rapid degradation of Glucagon-like peptide 1 (GLP-1) that is required for the secretion of insulin. DPP-4 also influences activation of node like receptor family, pyrin domain containing 3 (NLRP3) inflammasome under diabetic conditions. Although several polyphenols are reported for various bioactivities, they are consumed as part of the food matrix and not in isolation. Horsegram (Macrotyloma uniflorum) is a rich source of myricetin (Myr) (35 μg/g flour), reported for its anti-hyperglycemic effect. In this investigation, we aimed to study the effect of Myr, singly, and in the presence of co-nutrient horsegram protein (HP) on DPP-4 activity and its consequential impact on GLP-1, insulin, and NLRP3 inflammasome in high-fat diet and single low dose streptozotocin (STZ)-induced diabetic male Wistar rats. In diabetic control (DC), the activity of DPP-4 and its expression were higher compared to treated groups. The consequential decrease in the circulating GLP-1 levels in the DC group, but not treated groups, further indicated the effectiveness of our test molecules under diabetic conditions. Specifically, Myr decreased DPP-4 activity and its expression levels with enhanced circulating GLP-1 and insulin levels. Myr administration also resulted in a lessening of diabetes-induced NLRP3 inflammasome activation and enhanced antioxidant enzyme activities. HP also proved to be efficient in reducing elevated blood glucose levels and enhancing antioxidant enzyme activities. However, Myr, in the presence of HP as a co-nutrient, had diminished capacity to inhibit DPP-4 and, consequently, reduced potential in ameliorating diabetic conditions. Myr proved to be a potent inhibitor of DPP-4 in vitro and in vivo, resulting in enhanced circulating GLP-1 and insulin levels, thereby improving diabetic conditions. Though Myr and HP, individually ameliorate diabetic conditions, their dietary combination had reduced efficiency.
Databáze: OpenAIRE
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