A functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigs
| Autor: | Sang-Hyun Han, Yuliaxis Ramayo-Caldas, In-Cheol Cho, Chae-Kyoung Yoo, Hyun-Sook Shin, Youn-Chul Ryu, Jun Heon Lee, Hee-Bok Park, Wu-Sheng Sun, Dong-Hwan Kim, Yong-Jun Kang, Beom-Young Park, Hyun-Tae Lim, Yoo-Kyung Kim, Moon-Suck Ko, Seongsoo Hwang, Jin Seop Ahn, Göran Andersson, Pil-Nam Seong, Kichoon Lee, Sang-Geum Kim, Sung-Soo Lee, Eun-Ji Jung, Miguel Pérez-Enciso, Jae-Bong Lee, Jeong-Woong Lee, In-Sul Hwang |
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| Přispěvatelé: | Korea Research Institute of Bioscience & Biotechnology, Government of South Korea, Ministerio de Economía y Competitividad (España), Producció Animal, Genètica i Millora Animal, National Institute of Animal Science, Kongju National University, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Jeju National University (KOREA), Chonbuk National University, Gyeongsang National University, Bio-Medical Science Co, University of Science and Technology, Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Institute of Agrifood Research and Technology (IRTA), Chungnam National University (CNU), The Ohio State University Press, Swedish University of Agricultural Sciences (SLU), Centre for Research in Agricultural Genomics (CRAG), Universitat Autònoma de Barcelona (UAB), Institució Catalana de Recerca i Estudis Avançats (ICREA), Cooperative Research Program for Agriculture Science Technology Development (Project title: A research on performance improvement of `Nanchukmacdon', a high quality pig breed developed using Korean native black pigs (KNP) in Jeju Island' Rural Developme PJ01012301 PJ01262701, Korea Research Institute of Bioscience and Biotechnology (KRIBB) Research Initiative Program, Republic of Korea, Ministerio de Economia y Competitividad, Spain AGL2016-78709-R |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Cancer Research
Heredity Swine [SDV]Life Sciences [q-bio] Muscle Fibers Skeletal Sus scrofa QH426-470 Breeding MyoD Biochemistry Database and Informatics Methods Mice 0302 clinical medicine Animal Products Animal Cells Myosin Medicine and Health Sciences Myocyte Musculoskeletal System Genetics (clinical) Connective Tissue Cells 2. Zero hunger Genetics Mammals 0303 health sciences Adipogenesis [SDV.BA]Life Sciences [q-bio]/Animal biology Muscles Eukaryota Agriculture Genomics Enzymes Genetic Mapping medicine.anatomical_structure Adipose Tissue Connective Tissue Vertebrates MYF5 Intramuscular fat Anatomy Cellular Types Oxidoreductases Sequence Analysis Luciferase Research Article 663/664 Meat Genotype Bioinformatics Variant Genotypes Mice Transgenic Biology Myosins Research and Analysis Methods 03 medical and health sciences Sequence Motif Analysis medicine Genome-Wide Association Studies Animals Allele Nucleotide Motifs Muscle Skeletal Molecular Biology Ecology Evolution Behavior and Systematics 030304 developmental biology Nutrition [SDV.GEN]Life Sciences [q-bio]/Genetics Myosin Heavy Chains Organisms Skeletal muscle Biology and Life Sciences Computational Biology Proteins Human Genetics Cell Biology Fibroblasts Genome Analysis Diet Cytoskeletal Proteins Biological Tissue Skeletal Muscles Gene Expression Regulation Food Myogenic regulatory factors Amniotes Enzymology 030217 neurology & neurosurgery Genome-Wide Association Study |
| Zdroj: | Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona PLoS Genetics, Vol 15, Iss 10, p e1008279 (2019) PLoS Genetics Digital.CSIC. Repositorio Institucional del CSIC instname PLoS Genetics, Public Library of Science, 2019, 15 (10), pp.e1008279. ⟨10.1371/journal.pgen.1008279⟩ IRTA Pubpro. Open Digital Archive Institut de Recerca i Tecnologia Agroalimentàries (IRTA) |
| ISSN: | 1553-7390 1553-7404 |
| Popis: | Data Availability Statement: Full-length MYH3 CDS sequence from KNP, KX538787; full-length MYH3 CDS sequence from Landrace, KX538788. Two-kb of 5’-UTR MYH3 genomic DNA sequence from KNP, KX549312; 0.5-kb of 3’-UTR MYH3 genomic DNA sequence from KNP, KX549313. Two-kb of 5’-UTR MYH3 genomic DNA sequence from Landrace, KX549311; 0.5-kb of 3’-UTR MYH3 genomic DNA sequence from Landrace, KX549314. Resequencing data access information is provided in S10 Table. All the raw data to produce figures and tables are available at https://datadryad.org/review?doi=doi:10.5061/dryad.dr32n87 Muscle development and lipid accumulation in muscle critically affect meat quality of livestock. However, the genetic factors underlying myofiber-type specification and intramuscular fat (IMF) accumulation remain to be elucidated. Using two independent intercrosses between Western commercial breeds and Korean native pigs (KNPs) and a joint linkage-linkage disequilibrium analysis, we identified a 488.1-kb region on porcine chromosome 12 that affects both reddish meat color (a*) and IMF. In this critical region, only the MYH3 gene, encoding myosin heavy chain 3, was found to be preferentially overexpressed in the skeletal muscle of KNPs. Subsequently, MYH3-transgenic mice demonstrated that this gene controls both myofiber-type specification and adipogenesis in skeletal muscle. We discovered a structural variant in the promotor/regulatory region of MYH3 for which Q allele carriers exhibited significantly higher values of a* and IMF than q allele carriers. Furthermore, chromatin immunoprecipitation and cotransfection assays showed that the structural variant in the 5'-flanking region of MYH3 abrogated the binding of the myogenic regulatory factors (MYF5, MYOD, MYOG, and MRF4). The allele distribution of MYH3 among pig populations worldwide indicated that the MYH3 Q allele is of Asian origin and likely predates domestication. In conclusion, we identified a functional regulatory sequence variant in porcine MYH3 that provides novel insights into the genetic basis of the regulation of myofiber type ratios and associated changes in IMF in pigs. The MYH3 variant can play an important role in improving pork quality in current breeding programs. This work was carried out with the support of >Cooperative Research Program for Agriculture Science & Technology Development (Project title: A research on performance improvement of ‘Nanchukmacdon’, a high quality pig breed developed using Korean native black pigs (KNP) in Jeju Island, Project No. PJ01012301 and PJ01262701)> Rural Development Administration (http://www.rda.go.kr), Republic of Korea. This work was also supported by the Korea Research Institute of Bioscience and Biotechnology (KRIBB) Research Initiative Program, Republic of Korea (www.kribb.re.kr). M.P.E work was funded by AGL2016-78709-R (Ministerio de Economía y Competitividad, Spain; http://www.mineco.gob.es/portal/site/mineco/). T |
| Databáze: | OpenAIRE |
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