Development and verification of real-time PCR assay for identification of viral agents causing acute respiratory infections in human beings
| Autor: | A. V. Demina, Vladimir A. Ternovoi, Alexander P. Agafonov, Sergeev An, O. K. Demina, E. I. Sergeeva, A. N. Shikov, S. A. Beryllo, Denis V. Korneev |
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| Rok vydání: | 2013 |
| Předmět: |
diagnosis of respiratory infections
viruses acute respiratory infection (ARI) Biology Microbiology Genome Virus chemistry.chemical_compound Equivalent Virology Complementary DNA Pandemic Multiplex polymerase chain reaction Genetics Experimental Works flu virus Molecular Biology virus diseases adenovirus multiplex PCR respiratory-syncytial virus respiratory tract diseases Infectious Diseases Real-time polymerase chain reaction chemistry DNA |
| Zdroj: | Molecular Genetics, Microbiology and Virology |
| ISSN: | 1934-841X 0891-4168 |
| DOI: | 10.3103/s0891416813040083 |
| Popis: | A multiplex polymerase chain reaction (PCR) for identification of four viruses causing acute respiratory diseases in human beings was developed. The analytical sensitivity of developed RT-PCR for identification of adenovirus, respiratory-syncytial virus, flu viruses types A and B, and actual subtypes of type A flu virus (seasonal and pandemic variants H1N1, seasonal H3N2, and viruses of bird flu that are pathogenic to human beings H5 and H7) was 1 × 103 genome equivalents per milliliter. Diagnostic sensitivity for flu virus type A and B, and also subtypes H1 (seasonal H1N1, pandemic variant of H1N1 of year 2009), H3, H5 was 1 × 103–104 viral particles per milliliter. The method developed has high specificity and does not have positive signal in experiments with DNA/cDNA of human beings and viral DNA. We have studied 50 samples using the developed set. Etiology was defined in 33 samples. |
| Databáze: | OpenAIRE |
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