The Role of Guanine Nucleotide Binding Proteins in Hamsters with Myocardial Hypertrophy
| Autor: | Juro Yamamoto, Tadaaki Iwasaki, Kiyoko Nakamura, Jun Shibuya, Mitsumasa Ohyanagi |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Male
medicine.medical_specialty Guanine G protein Blotting Western Cardiomegaly Biology Left ventricular hypertrophy Biochemistry DNA-binding protein Pathogenesis chemistry.chemical_compound Western blot GTP-Binding Proteins Cricetinae Internal medicine medicine Animals RNA Messenger Molecular Biology Pressure overload Mesocricetus medicine.diagnostic_test Body Weight Organ Size Cell Biology Blotting Northern medicine.disease Blot Endocrinology chemistry Adenylyl Cyclases |
| Zdroj: | Journal of Receptors and Signal Transduction. 16:225-242 |
| ISSN: | 1532-4281 1079-9893 |
| Popis: | To clarify the role of the guanine nucleotide binding proteins (G-proteins) in the pathogenesis of myocardial hypertrophy, we investigated the alterations in myocardial G proteins in 20-week-old F1b hamsters with pressure overload induced for 3 days (3-day AS), 7 days (7-day AS) and 14 days (14-day AS) by the stenosis of the abdominal aorta, and in 4- and 20 week-old BIO 14.6 Syrian hamsters (4-wk and 20-wk BIO) with genetic myocardial hypertrophy. The hearts of 7-day AS, 14-day AS and 20-wk BIO with left ventricular hypertrophy exhibited a decrease in the mRNA levels detected by Northern blot analysis and protein levels of G protein detected by Western blot analysis as compared with sham-operated and age-matched F1b hearts. The function of Gs or Gi showed a concomitant reduction in both models of myocardial hypertrophy. The hearts of 3-day AS and 4-wk BIO without myocardial hypertrophy showed no changes in G proteins as compared with sham-operated and age-matched F1b hearts. These results suggest that a decrease in G proteins is not involved in the pathogenesis of myocardial hypertrophy, but that myocardial hypertrophy reduced the G proteins. |
| Databáze: | OpenAIRE |
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