Allergen immunotherapy modulates sensitivity of Treg cells to apoptosis in a rat model of allergic asthma
| Autor: | Somnath Mondal, Prasanta Kumar Das, Santanu Kumar Tripathi, Sk Md Omar Faruk, Iman Hazra, Saibal Moitra, Ankur Datta, Swapna Chaudhuri |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Allergy Allergen immunotherapy medicine.medical_treatment Immunology chemical and pharmacologic phenomena Apoptosis medicine.disease_cause T-Lymphocytes Regulatory Fas ligand 03 medical and health sciences 0302 clinical medicine Allergen Immunology and Allergy Medicine Animals Humans Rats Wistar Administration Intranasal Cells Cultured biology business.industry Perforin FOXP3 Rhinitis Allergic Seasonal hemic and immune systems Forkhead Transcription Factors Immunotherapy Allergens Antigens Plant medicine.disease Rats Disease Models Animal 030104 developmental biology Oncology Granzyme Proto-Oncogene Proteins c-bcl-2 Desensitization Immunologic biology.protein Pollen business Alstonia 030215 immunology |
| Zdroj: | Immunotherapy. 9(15) |
| ISSN: | 1750-7448 |
| Popis: | Aim: To study the apoptosis of Foxp3+ Treg cells following Alstonia scholaris pollen sensitization–challenge and following allergen immunotherapy. Materials & methods: Wistar rats were sensitized–challenged with Alstonia scholaris pollen and were further given intranasal immunotherapy. For the analysis of the apoptotic proteins on Treg cells by flow cytometry, multiple gating procedures were followed. Results: Allergen sensitization–challenge increases Annexin-V, Fas, FasL, caspases-8, 9, 3 cytochrome-C, APAF-1, Bax, perforin-1 and granzyme-B on Treg cells which is decreased following intranasal immunotherapy. On the other hand, Bcl-2 expression is decreased in allergy and increased by immunotherapy. Conclusion: Apoptosis of Treg cells is increased following allergen sensitization–challenge via extrinsic, intrinsic and perforin/granzyme pathways and allergen immunotherapy decreased the sensitivity to apoptosis of Treg cells. |
| Databáze: | OpenAIRE |
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