Delineation of the minimal portion of the Bacillus sphaericus 1593M toxin required for the expression of larvicidal activity
| Autor: | Peter Sebo, Françoise de la Torre, Jekisiel Szulmajster, Thérèse Bennardo |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Proteases
Bacterial Toxins Blotting Western DNA Mutational Analysis Molecular Sequence Data Restriction Mapping Peptide Bacillus medicine.disease_cause Biochemistry Bacillus sphaericus Microbiology Gene product Structure-Activity Relationship Gene expression medicine Amino Acid Sequence Cloning Molecular Escherichia coli chemistry.chemical_classification biology Toxin biology.organism_classification Recombinant Proteins Amino acid chemistry Genes Bacterial |
| Zdroj: | European journal of biochemistry. 194(1) |
| ISSN: | 0014-2956 |
| Popis: | The two genes of Bacillus sphaericus 1953M coding for the 51.4-kDa and 41.9-kDa proteins are both required for the expression of the active larvicidal toxin in Escherichia coli. The minimal size of the active peptide of the 41.9-kDa toxin was defined by in vitro deletion analysis of the gene and found to consist of 338 amino acids (38.3 kDa). N-terminal deletions past the Ile18 residue and C-terminal deletions past the His352 residue result in the loss of toxic activity and rapid degradation of such modified toxins by host proteases. The minimal active 38.3-kDa peptide produced in E. coli seems to mimick the stable processed form of the toxin found in larval midguts. However, it still requires the action of the synergistic 51.4-kDa protein for the larvicidal activity. |
| Databáze: | OpenAIRE |
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