Design, preparation, and in vitro characterization of a trimodally-targeted nanomagnetic onco-theranostic system for cancer diagnosis and therapy
| Autor: | Somayeh Sadighian, Mehrdad Hamidi, Omidreza Firuzi, Abdolhossein Zarrin, Ramin Miri, Soliman Mohammadi-Samani, Kobra Rostamizadeh |
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| Rok vydání: | 2015 |
| Předmět: |
Theranostic Nanomedicine
Cell Survival MRI contrast agent Drug Compounding Pharmaceutical Science Nanotechnology 02 engineering and technology Nod 010402 general chemistry 01 natural sciences Chitosan chemistry.chemical_compound Mice Folic Acid In vivo Neoplasms medicine Animals Humans MTT assay Doxorubicin Magnetite Nanoparticles Antibiotics Antineoplastic Magnetic Phenomena Hyperthermia Induced Hydrogen-Ion Concentration 021001 nanoscience & nanotechnology 0104 chemical sciences Drug Liberation chemistry Folate receptor Drug Design Cancer research MCF-7 Cells NIH 3T3 Cells 0210 nano-technology medicine.drug Folic Acid Transporters |
| Zdroj: | International journal of pharmaceutics. 500(1-2) |
| ISSN: | 1873-3476 |
| Popis: | In this study, the aim was to introduce and characterize a new trimodally-targeted nanomagnetic onco-theranostic system for simultaneous early diagnosis and efficient treatment of cancer. The onco-theranostic system was designed as it could target the tumor site through three targeting approach, i.e. magnetic, folic acid receptor, and pH sensitivity, and concurrently, due to the presence of superparamagnetic iron oxide nanoparticles (SPIONs) with super paramagnetic characteristics could be useful as MRI contrast agent for early cancer diagnosis. To achieve this goal, SPIONs were coated with chitosan and folic acid-conjugated chitosan via ionic gelation method in order to obtain non-targeted nanomagnetic onco-diagnostic (NT/NOD) and targeted nanomagnetic onco-diagnostic (T/NOD) systems. Finally, doxorubicin was loaded successfully into NT/NOD and T/NOD in order to obtain nanomagnetic onco-theranostic (NT/NOT) and targeted nanomagnetic onco-theranostic (T/NOT) systems. The entrapment efficiency and drug loading of T/NOT was determined to be 62.33 ± 5.20% and 10.26 ± 1.36%, respectively. MTT assay revealed that all systems were biocompatible within the concentration range investigated. Also, the T/NOT system showed the lowest IC50 comparing with free doxorubicin and NT/NOT system. In addition, uptake studies and competitive inhibition study verified the folate receptor mediated endocytosis of targeted system by MCF-7 as a folate receptor-positive cell line. The finding revealed that the extent of drug release from theranostic systems was pH-sensitive as it was higher at acidic media compared to that of in the neutral condition. Finally, T2-weighted phantom images, with an acceptable and dose-dependent resolution, proved the potential of T/NOT system as promising T2 MR contrast agent for diagnostic purpose. These finding proved that the prepared T/NOT system have great potential as a novel tumor-targeting nanotheranostic agent for simultaneous MRI imaging and treatment of folate receptor-positive cancers. Further studies are needed to test their behavior in vivo. |
| Databáze: | OpenAIRE |
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