Molecular Characterization of Multidrug-Resistant Escherichia coli Isolated from Milk of Dairy Cows with Clinical Mastitis in Algeria
| Autor: | Menoueri M Nabil, Edgarthe Priscilla Ngaiganam, Tennah Safia, Seydina M. Diene, Zerrouki Hanane, Jean-Marc Rolain, Sedrati Tahar, Chadi Hafidha, Azzi Omar |
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| Přispěvatelé: | Laboratoire de Recherche Gestion des Ressources Animales Locales (GRAL), Ecole Nationale Supérieure Vétérinaire d’Alger, Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Tetracycline [SDV]Life Sciences [q-bio] 030106 microbiology Mastitis Microbial Sensitivity Tests Biology medicine.disease_cause Microbiology beta-Lactamases 03 medical and health sciences Antibiotic resistance Drug Resistance Multiple Bacterial Escherichia coli medicine Animals Humans Escherichia coli Infections Amoxicillin biochemical phenomena metabolism and nutrition medicine.disease Anti-Bacterial Agents 3. Good health Ciprofloxacin Multiple drug resistance Milk 030104 developmental biology Algeria Multilocus sequence typing Cattle Female Plasmids Food Science medicine.drug |
| Zdroj: | Journal of food protection Journal of food protection, 2020, 83 (12), pp.2173-2178. ⟨10.4315/JFP-20-198⟩ |
| DOI: | 10.4315/JFP-20-198⟩ |
| Popis: | The objective of this study was to investigate the occurrence of multidrug-resistant Escherichia coli in cows with clinical mastitis in 42 different dairy farms located in the Bordj Bou Arreridj region of Algeria. Milk samples were cultured on Columbia blood agar, and isolates were then identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry. In total, 200 samples were screened and 52 E. coli strains confirmed as causative agents were obtained. The antimicrobial susceptibility testing was performed by disk diffusion method. Antibiotic resistance genes, including those conferring resistance to extended-spectrum beta-lactamases (i.e., bla(TEM), bla(SHV), and bla(CTX-M)), tetracyclines (tetA, tetB, tetC, and tetJ), aminoglycosides [aph(30), aac(30), aac(60), ant, aad, and armA], and quinolones (qnrA and qnrB) were amplified by standard PCR and sequenced when positive. Transferability of resistance genes has been investigated by conjugation experiments and multilocus sequence typing. The most frequently observed resistance was to amoxicillin (86.5%), followed by tetracycline (75%), amoxicillin-clavulanic acid (59.6%), trimethoprim-sulfamethoxazole (36.5%), doxycycline (13.5%), and ciprofloxacin (13.5%). Multidrug resistance was observed in 38.4% of isolates. Genotypic characterization showed that tetA (44.2%) and bla(TEM-1) (30.7%) genes were the most prevalent. Screening for plasmid-mediated quinolone resistance genes demonstrated that seven isolates (13.5%) expressed qnrB and one isolate (1.9%) harbored qnrA. In addition, aminoglycoside resistance determinants including aadA1 and aac(3)-Id were detected in seven and two isolates, respectively. Moreover, blaTEM, tetA, tetB, qnrB, and aadA1 were successfully transferred horizontally to transconjugant strains. The multilocus sequence typing revealed the presence of three different sequence types (ST162, ST371, and ST 949). |
| Databáze: | OpenAIRE |
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