Increases of microRNA let-7e in peripheral blood mononuclear cells in Hashimoto's disease
| Autor: | Mikio Watanabe, Minori Saeki, Yoshinori Iwatani, Hiroshi Otsu, Naoya Inoue, Yuka Katsumata, Yukina Takuse, Tomoya Kagawa |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male medicine.medical_specialty Hashimoto's disease Endocrinology Diabetes and Metabolism Hashimoto Disease Peripheral blood mononuclear cell Pathogenesis 03 medical and health sciences Young Adult Endocrinology Internal medicine microRNA medicine Humans Cells Cultured Aged Aged 80 and over Messenger RNA business.industry Thyroid Interleukin Middle Aged medicine.disease Interleukin-10 Up-Regulation Gene Expression Regulation Neoplastic Interleukin 10 MicroRNAs 030104 developmental biology medicine.anatomical_structure Case-Control Studies Immunology Leukocytes Mononuclear Female business |
| Zdroj: | Endocrine journal. 63(4) |
| ISSN: | 1348-4540 |
| Popis: | MicroRNA (miRNA) is a family of non-coding RNAs that have important roles in various vital functions. It has been reported that let-7e, a miRNA, may be involved in the regulation of interleukin (IL)-10 production. The purpose of this study was to evaluate the role of let-7e as a regulator of IL-10 production in the pathological processes of autoimmune thyroid diseases (AITDs). We evaluated the association between let-7e expression and intracellular expression of IL-10 in the peripheral blood mononuclear cells (PBMCs) collected from 11 healthy volunteers. Then we investigated the expression levels of let-7e in the PBMCs of 50 patients with Graves' disease (GD), 42 patients with Hashimoto's disease (HD) and 28 healthy controls. We found negative correlations between the expression level of let-7e and IL-10 messengerRNA (mRNA) and between the expression level of let-7e and proportion of IL-10(+) cells in stimulated PBMCs from healthy volunteers (r = -0.44, p = 0.0267 and r = -0.49, p = 0.0166, respectively). The expression levels of let-7e were significantly increased in HD patients compared with those in GD patients and healthy volunteers (p = 0.0003 and p = 0.0011, respectively). let-7e may be associated with the pathogenesis of HD through the regulation of intracellular IL-10 expression. |
| Databáze: | OpenAIRE |
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