Release of low density lipoprotein receptors from human fibroblasts or Hela cells by tryptic digestion
| Autor: | Nicola Di Ferrante, Daniela T. Di Ferrante, Patricia V. Donnelly, Antonio M. Gotto, Salvatore Toma |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Receptors
Drug Mutant Biophysics Biochemistry Chromatography Affinity Cell Line HeLa chemistry.chemical_compound Affinity chromatography medicine Humans Trypsin Receptor Molecular Biology biology Cell Biology Fibroblasts biology.organism_classification Molecular biology Protamine Lipoproteins LDL Agglutination (biology) chemistry Low-density lipoprotein biology.protein lipids (amino acids peptides and proteins) HeLa Cells medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 84:366-373 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(78)90179-1 |
| Popis: | Trypsin treatment of cultured normal human skin fibroblasts or Hela cells releases material which is retained on a low density lipoprotein (LDL)-Sepharose affinity column, may be eluted from it with 2.5 M KI and, after dialysis, agglutinates LDL or apo-B-coated formocells. Such agglutination is prevented by preincubation of the receptor with LDL in solution or with arginine-rich protamine. Trypsin treatment of “receptor defective” or “receptor negative” mutant fibroblasts releases material which is retained on LDL-Sepharose column but fails to agglutinate LDL-coated formocells. The receptor may be labeled with 6-[ 3 H]-glucosamine·HCl and [ 3 H ]-leucine, it is inactivated by heating at 80°C for 10 min and may be obtained from normal fibroblasts or Hela cells, whether they were cultured in presence or in absence of lipoprotein-containing fetal calf serum. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|