Impression cytology implicates cell autophagy in aqueous deficiency dry eye
| Autor: | Joy Wang, Cindy M.L. Hutnik, Hong Liu, Richard Filek, Chong H Wu, Tony Lin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Programmed cell death genetic structures cell autophagy Cell impression cytology dry eye 03 medical and health sciences 0302 clinical medicine Ophthalmology LC3 Medicine Schirmer's test Ocular Surface Disease Index Original Research Frozen section procedure medicine.diagnostic_test business.industry Autophagy Impression cytology Clinical Ophthalmology technique eye diseases 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Biomarker (medicine) sense organs Schirmer’s test business |
| Zdroj: | Clinical Ophthalmology (Auckland, N.Z.) |
| ISSN: | 1177-5483 |
| Popis: | Tony Lin,1,2,* Richard Filek,3,* Joy M Wang,4 Chong H Wu,4 Hong Liu,2 Cindy ML Hutnik1–3 1Department of Ophthalmology, Western University, 2Ivey Eye Institute, St Joseph’s Health Care, StJoseph’s Hospital, 3Department of Pathology and Laboratory Medicine, 4Department of Biology, Western University, London, ON, Canada *These authors contributed equally to this work Purpose: Impression cytology in combination with a cell autophagy marker was used to demonstrate the utility of a novel frozen section technique, to assess the health of the ocular surface, as well as to correlate autophagic cell death with the commonly used clinical tests for dry eye. Methods: Female patients over the age of 18 years who attended an outpatient ophthalmology clinic were invited to participate. Schirmer’s test, tear film break-up time and the ocular surface disease index questionnaire were used as objective and subjective clinical tests for dry eye. The cellular biomarker microtubule-associated protein light chain 3 (LC3) was used as a marker of autophagic cell death. Results: Low LC3 nuclear staining was positively correlated with Schirmer’s test |
| Databáze: | OpenAIRE |
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