β-Lactamase Production in Prevotella intermedia, Prevotella nigrescens, and Prevotella pallens Genotypes and In Vitro Susceptibilities to Selected Antimicrobial Agents
| Autor: | Kari Salminen, Sirkka Asikainen, Jaana Mättö, Eija Könönen, Hannele Jousimies-Somer, Marja-Liisa Väisänen, Maria Saarela, Birgitta von Troil-Lindén, Sydney M. Finegold |
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| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Genotype
Population Microbial Sensitivity Tests Polymerase Chain Reaction Prevotella intermedia beta-Lactamases Microbiology 03 medical and health sciences 0302 clinical medicine Prevotella nigrescens medicine Humans Pharmacology (medical) Cefoxitin education Phylogeny Antibacterial agent Pharmacology 0303 health sciences education.field_of_study biology 030306 microbiology 030206 dentistry biology.organism_classification DNA Fingerprinting 3. Good health Anti-Bacterial Agents Penicillin Trovafloxacin stomatognathic diseases Infectious Diseases DNA profiling Susceptibility medicine.drug |
| Zdroj: | Scopus-Elsevier Mättö, J, Asikainen, S, Väisänen, M-L, Von Troil-Lindén, B, Könönen, E, Saarela, M, Salminen, K, Finegold, S M & Jousimies-Somer, H 1999, ' β-Lactamase Production in Prevotella intermedia, Prevotella nigrescens, and Prevotella pallens Genotypes and In Vitro Susceptibilities to Selected Antimicrobial Agents ', Antimicrobial Agents and Chemotherapy, vol. 43, no. 10, pp. 2383-2388 . https://doi.org/10.1128/AAC.43.10.2383 |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/AAC.43.10.2383 |
| Popis: | The present study investigated the β-lactamase production of 73 Prevotella intermedia , 84 Prevotella nigrescens , and 14 Prevotella pallens isolates and their in vitro susceptibilities to six antimicrobial agents. The P. intermedia and P. nigrescens isolates were recovered from oral and extraoral samples obtained from subjects in two geographic locations from 1985 to 1995. The clonality of the β-lactamase-positive and β-lactamase-negative isolates and the clustering of the genotypes were studied by arbitrarily primed-PCR fingerprinting. β-Lactamase production was detected in 29% of P. intermedia isolates, 29% of P. nigrescens isolates, and 57% of P. pallens isolates. No difference in the frequencies of β-lactamase production by P. intermedia and P. nigrescens between isolates from oral and extraoral sites, between isolates obtained at different time periods, or between P. intermedia isolates from different geographic locations was observed. However, the P. nigrescens isolates from the United States were significantly more frequently ( P = 0.015) β-lactamase positive than those from Finland. No association between the genotypes and β-lactamase production or between the genotypes and the sources of the isolates was found. The penicillin G MICs at which 90% of the isolates were inhibited were 8 μg/ml for P. intermedia , 8 μg/ml for P. nigrescens , and 16 μg/ml for P. pallens . For the β-lactamase-negative isolates, the corresponding values were 0.031, 0.031, and 0.125 μg/ml, and for the β-lactamase-positive isolates, the corresponding values were 16, 8, and 32 μg/ml. All isolates were susceptible to amoxicillin-clavulanate, cefoxitin, metronidazole, azithromycin, and trovafloxacin. The MICs of amoxicillin-clavulanate and cefoxitin were relatively higher for the β-lactamase-positive population than for the β-lactamase-negative population. |
| Databáze: | OpenAIRE |
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